ArticleBiology of reproduction2016
Mouse Spermatogenesis Requires Classical and Nonclassical Testosterone Signaling.
Article in Biology of reproduction, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 116 citations in OpenAlex.
- Androgen rapidly activates phosphorylation signaling pathways in Sertoli cells.Experimental and therapeutic medicine · 2026Article
- Extracellular vesicles in stem cell-based gonadal regeneration: mechanisms, therapeutic potential, and translational challenges.Frontiers in veterinary science · 2025Review
- Single-cell transcriptomic and cross-species comparison analyses reveal distinct molecular changes of porcine testes during puberty.Communications biology · 2024Article
- Single-Cell RNA Sequencing Reveals an Atlas of Hezuo Pig Testis Cells.International journal of molecular sciences · 2024Article
- Molecular insights into Sertoli cell function: how do metabolic disorders in childhood and adolescence affect spermatogonial fate?Nature communications · 2024Review
- Mechanisms of Male Reproductive Sterility Triggered by Dysbiosis of Intestinal Microorganisms.Life (Basel, Switzerland) · 2024Review
- Hormone Regulation in Testicular Development and Function.International journal of molecular sciences · 2024Review
- The gut microbiota improves reproductive dysfunction in obese mice by suppressing the NLRP3/ASC/caspase-1 axis.Future microbiology · 2024Article
- It puts the T's in fertility: testosterone and spermatogenesis.International journal of impotence research · 2022Review
- Cypermethrin inhibits proliferation of Sertoli cells through AR involving DAB2IP/PI3K/AKT signaling pathway in vitro.Toxicology research · 2022Article
- The Role of NLRP3 Inflammasome Activation and Oxidative Stress in Varicocele-Mediated Male Hypofertility.International journal of molecular sciences · 2022Review
- Gonadotropins treatment prior to microdissection testicular sperm extraction in non-obstructive azoospermia: a single-center cohort study.Reproductive biology and endocrinology : RB&E · 2022Article
- Serum- and Feeder-Free Culture of Juvenile Monkey Female Germline Stem Cells and Testosterone Regulation of their Self-Renewal.Stem cell reviews and reports · 2022Article
- Frozen-Thawed Sperm Analysis of Benign Prostatic Hyperplasia Dogs Treated With Finasteride.Frontiers in veterinary science · 2022Article
- What Does Androgen Receptor Signaling Pathway in Sertoli Cells During Normal Spermatogenesis Tell Us?Frontiers in endocrinology · 2022Review
- Declining levels of miR-382-3p at puberty trigger the onset of spermatogenesis.Molecular therapy. Nucleic acids · 2021Article
- The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited.International journal of molecular sciences · 2021Review
- Current markers for infertility in men.JBRA assisted reproduction · 2021Article
- Hormonal Treatment of Men with Nonobstructive Azoospermia: What Does the Evidence Suggest?Journal of clinical medicine · 2021Review
- Androgen Actions in the Testis and the Regulation of Spermatogenesis.Advances in experimental medicine and biology · 2021Review
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11 authors at 4 institutions in 1 country.
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Abstract
Testosterone acts though the androgen receptor in Sertoli cells to support germ cell development (spermatogenesis) and male fertility, but the molecular and cellular mechanisms by which testosterone acts are not well understood. Previously, we found that in addition to acting through androgen receptor to directly regulate gene expression (classical testosterone signaling pathway), testosterone acts through a nonclassical pathway via the androgen receptor to rapidly activate kinases that are known to regulate spermatogenesis. In this study, we provide the first evidence that nonclassical testosterone signaling occurs in vivo as the MAP kinase cascade is rapidly activated in Sertoli cells within the testis by increasing testosterone levels in the rat. We find that either classical or nonclassical signaling regulates testosterone-mediated Rhox5 gene expression in Sertoli cells within testis explants. The selective activation of classical or nonclassical signaling pathways in Sertoli cells within testis explants also resulted in the differential activation of the Zbtb16 and c-Kit genes in adjacent spermatogonia germ cells. Delivery of an inhibitor of either pathway to Sertoli cells of mouse testes disrupted the blood-testis barrier that is essential for spermatogenesis. Furthermore, an inhibitor of nonclassical testosterone signaling blocked meiosis in pubertal mice and caused the loss of meiotic and postmeiotic germ cells in adult mouse testes. An inhibitor of the classical pathway caused the premature release of immature germ cells. Collectively, these observations indicate that classical and nonclassical testosterone signaling regulate overlapping and distinct functions that are required for the maintenance of spermatogenesis and male fertility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.