ArticleBMC genomics2015
Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei).
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Functional Characterization of Tret1-like Gene and Its Critical Role in Regulating Growth inInternational journal of molecular sciences · 2026Article
- Imagin: An Integrase-Like Gene Conserved Across Malacostracan Crustaceans Derived From a Ginger1 DNA Transposon.Genome biology and evolution · 2026Article
- Full-Length Transcriptome of Testis and Ovary Provides Insights into Alternative Splicing During Gonadal Development inInternational journal of molecular sciences · 2025Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelid Platynereis dumerilii.Development (Cambridge, England) · 2025Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelidbioRxiv : the preprint server for biology · 2024Article
- Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management ofAnimals : an open access journal from MDPI · 2024Article
- Characterization of microsatellite markers in the coding regions of the Penaeus vannamei genome.PloS one · 2024Article
- Microbes and pathogens associated with shrimps - implications and review of possible control strategies.Frontiers in Marine Science · 2024Article
- Transcriptome profiling of banana shrimp (Fenneropenaeus merguiensis) ovaries and testes: Insights into FoxL2.PloS one · 2023Article
- Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis).PloS one · 2023Article
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- Two Short Repeats in the 5' Untranslated Region of Insulin-like Androgenic Gland Factor inInternational journal of molecular sciences · 2022Article
- Interactions Between Temperature Variability and Reproductive Physiology Across Traits in an Intertidal Crab.Frontiers in physiology · 2022Article
- Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn,Frontiers in genetics · 2022Article
- Comparative Analysis of PacBio and Oxford Nanopore Sequencing Technologies for Transcriptomic Landscape Identification ofLife (Basel, Switzerland) · 2021Article
- Transcriptome analysis of five ovarian stages reveals gonad maturation in female Macrobrachium nipponense.BMC genomics · 2021Article
- Expression dynamics of solute carrier family 15 member 4 (SLC15A4) and its potential regulatory role in ovarian development of the Indian white shrimp, Penaeus indicus.Molecular biology reports · 2020Article
- Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming CrabFrontiers in endocrinology · 2020Article
- Article
- De novo gonad transcriptome analysis of the common littoral shrimp Palaemon serratus: novel insights into sex-related genes.BMC genomics · 2019Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Pacific white shrimp (Litopenaeus vannamei) is the world's most prevalent cultured crustacean species. However, the supply of high-quality broodstocks is limited and baseline information related to its reproductive activity and molecular issues related to gonad development are scarce. In this study, we performed transcriptome sequencing on the gonads of adult male and female L. vannamei to identify sex-related genes.
resultsA total of 25.16 gigabases (Gb) of sequences were generated from four L. vannamei gonadal tissue libraries. After quality control, 24.11 Gb of clean reads were selected from the gonadal libraries. De-novo assembly of all the clean reads generated a total of 65,218 unigenes with a mean size of 1021 bp and a N50 of 2000 bp. A search of all-unigene against Nr, SwissProt, KEGG, COG and NT databases resulted in 26,482, 23,062, 20,659, 11,935 and 14,626 annotations, respectively, providing a total of 30,304 annotated unigenes. Among annotated unigenes, 12,320 unigenes were assigned to gene ontology categories and 20,659 unigenes were mapped to 258 KEGG pathways. By comparing the ovary and testis libraries, 19,279 testicular up-regulated and 3,529 ovarian up-regulated unigenes were identified. Enrichment analysis of differentially expressed unigenes resulted in 1060 significantly enriched GO terms and 34 significantly enriched KEGG pathways. Nine ovary-specific, 6 testis-specific, 45 testicular up-regulated and 39 ovarian up-regulated unigenes were then confirmed by semi-quantitative PCR and quantitative real-time PCR. In addition, using all-unigenes as a reference, a total of 13,233 simple sequence repeats (SSRs) were identified in 10,411 unigene sequences.
conclusionsThe present study depicts the first large-scale RNA sequencing of shrimp gonads. We have identified many important sex-related functional genes, GO terms and pathways, all of which will facilitate future research into the reproductive biology of shrimp. We expect that the SSRs detected in this study can then be used as genetic markers for germplasm evaluation of breeding and imported populations.
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