Evidence map›Paper›PMID 26607451›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis.

Chunhua Zhang, Michelle Q Brown, Wilhelmina van de Ven, Zhi-Min Zhang, Bin Wu, Michael C Young, Lukáš Synek, Dan Borchardt, Reed Harrison, Songqin Pan and 16 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.

0numbers the graph read from it
0cells of the map it votes in
93citing papers in PubMed
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

93 citing papers in PubMed, 152 citations in OpenAlex.

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  9. Preassembly and independent trafficking of the exocyst complex inProceedings of the National Academy of Sciences of the United States of America · 2025
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33 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

26 authors at 5 institutions in 2 countries.

Chunhua ZhangCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Michelle Q BrownCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Wilhelmina van de VenCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Zhi-Min ZhangDepartment of Biochemistry, University of California, Riverside, CA 92521;
Bin WuDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19104;
Michael C YoungDepartment of Chemistry, University of California, Riverside, CA 92521;
Lukáš SynekInstitute of Experimental Botany, Academy of Sciences, 165 02 Prague 6, Czech Republic;
Dan BorchardtDepartment of Chemistry, University of California, Riverside, CA 92521;
Reed HarrisonDepartment of Bioengineering, University of California, Riverside, CA, 92521;
Songqin PanCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Nan LuoCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Yu-Ming M HuangDepartment of Chemistry, University of California, Riverside, CA 92521;
Yoo-Jin GhangDepartment of Chemistry, University of California, Riverside, CA 92521;
Nolan UngCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Ruixi LiCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Jonathan IsleyDepartment of Biology, Wake Forest University, Winston-Salem, NC 27109; Center for Molecular Communication and Signaling, Wake Forest University, Winston-Salem, NC 27109;
Dimitrios MorikisDepartment of Bioengineering, University of California, Riverside, CA, 92521;
Jikui SongDepartment of Biochemistry, University of California, Riverside, CA 92521;
Wei GuoDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19104;
Richard J HooleyDepartment of Chemistry, University of California, Riverside, CA 92521;
Chia-En A ChangDepartment of Chemistry, University of California, Riverside, CA 92521;
Zhenbiao YangCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Viktor ZarskyInstitute of Experimental Botany, Academy of Sciences, 165 02 Prague 6, Czech Republic; Department of Experimental Plant Biology, Faculty of Sciences, Charles University, 128 43 Prague 2, Czech Republic.
Gloria K MudayDepartment of Biology, Wake Forest University, Winston-Salem, NC 27109; Center for Molecular Communication and Signaling, Wake Forest University, Winston-Salem, NC 27109;
Glenn R HicksCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
Natasha V RaikhelCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521; natasha.raikhel@ucr.edu.
University of California, Riverside · USUniversity of Pennsylvania · USWake Forest University · USCharles University · CZCzech Academy of Sciences, Institute of Experimental Botany · CZ

Funding

Molecular Regulation of ExocytosisR01GM111128 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI GUO, WEI · 2014 to 2020
$2.4M
NIGMS NIH HHS R01 GM111128
6 · The paper itself

Abstract

The exocyst complex regulates the last steps of exocytosis, which is essential to organisms across kingdoms. In humans, its dysfunction is correlated with several significant diseases, such as diabetes and cancer progression. Investigation of the dynamic regulation of the evolutionarily conserved exocyst-related processes using mutants in genetically tractable organisms such as Arabidopsis thaliana is limited by the lethality or the severity of phenotypes. We discovered that the small molecule Endosidin2 (ES2) binds to the EXO70 (exocyst component of 70 kDa) subunit of the exocyst complex, resulting in inhibition of exocytosis and endosomal recycling in both plant and human cells and enhancement of plant vacuolar trafficking. An EXO70 protein with a C-terminal truncation results in dominant ES2 resistance, uncovering possible distinct regulatory roles for the N terminus of the protein. This study not only provides a valuable tool in studying exocytosis regulation but also offers a potentially new target for drugs aimed at addressing human disease.

Indexed as

ExocytosisArabidopsisArabidopsis ProteinsCell MembraneConserved SequenceEndosomesEvolution, MolecularHumansLimoninsProtein Structure, SecondaryArabidopsis Proteinsendosidin 2EXO70A1 protein, ArabidopsisLimoninsendosidin2EXO70exocystexocytosis

Identifiers

PMID26607451
PMCPMC4711834
OpenAlexW2177524008

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.