Evidence map›Paper›PMID 26607010›Full record

ArticleBiochimica et biophysica acta2016

17β-estradiol (E2) in membranes: Orientation and dynamic properties.

Jason J Guo, De-Ping Yang, Xiaoyu Tian, V Kiran Vemuri, Dali Yin, Chen Li, Richard I Duclos, Lingling Shen, Xiaoyu Ma, David R Janero and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Jason J GuoCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA. Electronic address: j.guo@neu.edu.
De-Ping YangPhysics Department, College of the Holy Cross, 1 College Street, Worcester, MA 01610, USA.
Xiaoyu TianCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
V Kiran VemuriCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
Dali YinCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
Chen LiCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
Richard I DuclosCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
Lingling ShenCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
Xiaoyu MaCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
David R JaneroCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA.
Alexandros MakriyannisCenter for Drug Discovery, Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA; Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115-5000, USA. Electronic address: a.makriyannis@neu.edu.
Northeastern University · USCollege of the Holy Cross · US

Funding

MOLECULAR BASIS OF CANNABINOID ACITIVITYR37DA003801 · NIDA · UNIVERSITY OF CONNECTICUT STORRS · PI MAKRIYANNIS, ALEXANDROS · 1997 to 2006
$3.7M
CANNABINOID LIGANDS AND DRUGSR01DA007215 · NIDA · UNIVERSITY OF CONNECTICUT STORRS · PI MAKRIYANNIS, ALEXANDROS · 1993 to 2012
$3.6M
MOLECULAR BASIS OF CANNABINOID ACTIVITYR01DA003801 · NIDA · UNIVERSITY OF CONNECTICUT STORRS · PI MAKRIYANNIS, ALEXANDROS · 1985 to 2017
$2.5M
Anandamide Carrier Proteins as Drug TargetsR33DA032020 · NIDA · NORTHEASTERN UNIVERSITY · PI GUO, JASON JIANXIN · 2014 to 2015
$767k
Anandamide Carrier Proteins as Drug TargetsR21DA032020 · NIDA · NORTHEASTERN UNIVERSITY · PI GUO, JASON JIANXIN · 2012 to 2013
$274k
NIDA NIH HHS DA032020NIDA NIH HHS DA3801NIDA NIH HHS DA7215NIDA NIH HHS R01 DA003801NIDA NIH HHS R01 DA007215NIDA NIH HHS R21 DA032020NIDA NIH HHS R33 DA032020NIDA NIH HHS R37 DA003801
6 · The paper itself

Abstract

Non-genomic membrane effects of estrogens are of great interest because of the diverse biological activities they may elicit. To further our understanding of the molecular features of the interaction between estrogenic hormones and membrane bilayers, we have determined the preferred orientation, location, and dynamic properties of 17β-estradiol (E2) in two different phospholipid membrane environments using (2)H-NMR and 2D (1)H-(13)C HSQC in conjunction with molecular dynamics simulations. Unequivocal spectral assignments to specific (2)H labels were made possible by synthesizing six selectively deuterated E2 molecules. The data allow us to conclude that the E2 molecule adopts a nearly "horizontal" orientation in the membrane bilayer with its long axis essentially perpendicular to the lipid acyl-chains. All four rings of the E2 molecule are located near the membrane interface, allowing both the E2 3-OH and the 17β-OH groups to engage in hydrogen bonding and electrostatic interactions with polar phospholipid groups. The findings augment our knowledge of the molecular interactions between E2 and membrane bilayer and highlight the asymmetric nature of the dynamic motions of the rigid E2 molecule in a membrane environment.

Indexed as

Membranes, ArtificialMolecular Dynamics SimulationEstradiolEstradiolMembranes, ArtificialBiomembranesDrugsEstrogenNMRPhospholipidsRelaxation agents

Identifiers

PMID26607010
PMCPMC4703515
OpenAlexW2182921419

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.