ReviewImmunology and cell biology2016
Clonal expansion under the microscope: studying lymphocyte activation and differentiation using live-cell imaging.
Review in Immunology and cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Benchmarking the prediction of responding cells to perturbations affecting both gene expression and cellular abundance using scRNA sequencing.Scientific reports · 2026Article
- A Modular In-Incubator Microscope for Longitudinal Live Cell Microscopy.bioRxiv : the preprint server for biology · 2026Article
- A Quantitative Printability Framework for Programmable Assembly of Pre-Vascular Patterns via Laser-Induced Forward Transfer.Advanced healthcare materials · 2026Article
- Independent and temporally separated dynamics for RORγt and Foxp3 during Th17 differentiation.Frontiers in immunology · 2025Article
- Time-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation.Communications biology · 2023Article
- Best practices for single-cell analysis across modalities.Nature reviews. Genetics · 2023Review
- Heritable changes in division speed accompany the diversification of single T cell fate.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Dissecting the dynamic transcriptional landscape of early T helper cell differentiation into Th1, Th2, and Th1/2 hybrid cells.Frontiers in immunology · 2022Article
- Advances in antibody discovery from human BCR repertoires.Frontiers in bioinformatics · 2022Review
- A Single-Cell Perspective on Memory T-Cell Differentiation.Cold Spring Harbor perspectives in biology · 2021Review
- Assay and Isolation of Single Proliferating CD4+ Lymphocytes Using an Automated Microraft Array Platform.IEEE transactions on bio-medical engineering · 2020Article
- Stochastic Inheritance of Division and Death Times Determines the Size and Phenotype of CD8Frontiers in immunology · 2019Article
- Article
- Nongenetic origins of cell-to-cell variability in B lymphocyte proliferation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- In vitro tracking and intracellular protein distribution in immunology.Immunology and cell biology · 2017Review
- Autonomous TNF is critical for in vivo monocyte survival in steady state and inflammation.The Journal of experimental medicine · 2017Article
- Cutting-edge single-cell genomics and modelling in immunology.Immunology and cell biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clonal expansion of lymphocytes is a hallmark of vertebrate adaptive immunity. A small number of precursor cells that recognize a specific antigen proliferate into expanded clones, differentiate and acquire various effector and memory phenotypes, which promote effective immune responses. Recent studies establish a large degree of heterogeneity in the level of expansion and in cell state between and within expanding clones. Studying these processes in vivo, while providing insightful information on the level of heterogeneity, is challenging due to the complex microenvironment and the inability to continuously track individual cells over extended periods of time. Live cell imaging of ex vivo cultures within micro fabricated arrays provides an attractive methodology for studying clonal expansion. These experiments facilitate continuous acquisition of a large number of parameters on cell number, proliferation, death and differentiation state, with single-cell resolution on thousands of expanding clones that grow within controlled environments. Such data can reveal stochastic and instructive mechanisms that contribute to observed heterogeneity and elucidate the sequential order of differentiation events. Intercellular interactions can also be studied within these arrays by following responses of a controlled number of interacting cells, all trapped within the same microwell. Here we describe implementations of live-cell imaging within microwell arrays for studies of lymphocyte clonal expansion, portray insights already gained from these experiments and outline directions for future research. These tools, together with in vivo experiments tracking single-cell responses, will expand our understanding of adaptive immunity and the ways by which it can be manipulated.
Indexed as
Identifiers
26606974What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.