Evidence map›Paper›PMID 26606532›Full record

ArticlePloS one2015

Ultrathin Polyimide Membrane as Cell Carrier for Subretinal Transplantation of Human Embryonic Stem Cell Derived Retinal Pigment Epithelium.

Tanja Ilmarinen, Hanna Hiidenmaa, Peeter Kööbi, Soile Nymark, Anni Sorkio, Jing-Huan Wang, Boris V Stanzel, Fabian Thieltges, Päivi Alajuuma, Olli Oksala and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Nano-Biomaterials for Retinal Regeneration.Nanomaterials (Basel, Switzerland) · 2021
    Review
  10. The LratInternational journal of molecular sciences · 2021
    Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Nanotechnology in regenerative ophthalmology.Advanced drug delivery reviews · 2019
    Review
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Tanja IlmarinenBioMediTech, University of Tampere, Tampere, Finland.
Hanna HiidenmaaBioMediTech, University of Tampere, Tampere, Finland.
Peeter KööbiDepartment of Ophthalmology, SILK, University of Tampere and Tays Eye Center, Tampere, Finland.
Soile NymarkDepartment of Electronics and Communications Engineering and BioMediTech, Tampere University of Technology, Tampere, Finland.
Anni SorkioBioMediTech, University of Tampere, Tampere, Finland.
Jing-Huan WangDepartment of Ophthalmology, SILK, University of Tampere and Tays Eye Center, Tampere, Finland.
Boris V StanzelDepartment of Ophthalmology, University of Bonn, Bonn, Germany.
Fabian ThieltgesDepartment of Ophthalmology, University of Bonn, Bonn, Germany.
Päivi AlajuumaSanten Oy, Tampere, Finland.
Olli OksalaSanten Oy, Tampere, Finland.
Marko KatajaTays Eye Center, Tampere, Finland.
Hannu UusitaloDepartment of Ophthalmology, SILK, University of Tampere and Tays Eye Center, Tampere, Finland.
Heli SkottmanBioMediTech, University of Tampere, Tampere, Finland.
Tampere University · FITampere University · FISanten (Finland) · FITampere University Hospital · FIUniversity of Bonn · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we investigated the suitability of ultrathin and porous polyimide (PI) membrane as a carrier for subretinal transplantation of human embryonic stem cell (hESC) -derived retinal pigment epithelial (RPE) cells in rabbits. The in vivo effects of hESC-RPE cells were analyzed by subretinal suspension injection into Royal College of Surgeons (RCS) rats. Rat eyes were analyzed with electroretinography (ERG) and histology. After analyzing the surface and permeability properties of PI, subretinal PI membrane transplantations with and without hESC-RPE were performed in rabbits. The rabbits were followed for three months and eyes analyzed with fundus photography, ERG, optical coherence tomography (OCT), and histology. Animals were immunosuppressed with cyclosporine the entire follow-up time. In dystrophic RCS rats, ERG and outer nuclear layer (ONL) thickness showed some rescue after hESC-RPE injection. Cells positive for human antigen were found in clusters under the retina 41 days post-injection but not anymore after 105 days. In rabbits, OCT showed good placement of the PI. However, there was loss of pigmentation on the hESC-RPE-PI over time. In the eyes with PI alone, no obvious signs of inflammation or retinal atrophy were observed. In the presence of hESC-RPE, mononuclear cell infiltration and retinal atrophy were observed around the membranes. The porous ultrathin PI membrane was well-tolerated in the subretinal space and is a promising scaffold for RPE transplantation. However, the rejection of the transplanted cells seems to be a major problem and the given immunosuppression was insufficient for reduction of xenograft induced inflammation.

Indexed as

Tissue ScaffoldsAnimalsCell LineDisease Models, AnimalElectroretinographyEpithelial CellsHuman Embryonic Stem CellsHumansRatsRetinal DegenerationRetinal Pigment EpitheliumTomography, Optical CoherenceTreatment Outcome

Identifiers

PMID26606532
PMCPMC4659637
OpenAlexW2176213108

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.