ArticleWater science and technology : a journal of the International Association on Water Pollution Research2015
Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring.
Article in Water science and technology : a journal of the International Association on Water Pollution Research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- Enhancing DNA recovery in low-biomass snow algae samples: a comparative study of extraction methods and their effect on community composition.Applied and environmental microbiology · 2026Article
- Assessing Freshwater Microbiomes from Different Storage Sources in the Caribbean Using DNA Metabarcoding.Microorganisms · 2023Article
- Waterborne pathogens detection technologies: advances, challenges, and future perspectives.Frontiers in microbiology · 2023Review
- Advancement of Metatranscriptomics towards Productive Agriculture and Sustainable Environment: A Review.International journal of molecular sciences · 2022Review
- Review of Nitrification Monitoring and Control Strategies in Drinking Water System.International journal of environmental research and public health · 2022Review
- ASAP 2: a pipeline and web server to analyze marker gene amplicon sequencing data automatically and consistently.BMC bioinformatics · 2022Article
- Enhancing diversity analysis by repeatedly rarefying next generation sequencing data describing microbial communities.Scientific reports · 2021Article
- Microbial diversity in full-scale water supply systems through sequencing technology: a review.RSC advances · 2021Review
- Bathing Water Quality Monitoring Practices in Europe and the United States.International journal of environmental research and public health · 2021Review
- The Ubiquity and Development-Related Abundance Dynamics ofMicroorganisms · 2021Article
- Diarrhea prevalence in a randomized, controlled prospective trial of point-of-use water filters in homes and schools in the Dominican Republic.Tropical medicine and health · 2021Article
- Evaluation of DNA extraction yield from a chlorinated drinking water distribution system.PloS one · 2021Article
- Physicochemical Parameters Affecting the Distribution and Diversity of the Water Column Microbial Community in the High-Altitude Andean Lake System of La Brava and La Punta.Microorganisms · 2020Article
- Article
- Geographical patterns of denitrifying bacterial communities associated with different urban lakes.RSC advances · 2018Article
- Investigation of Detection Limits and the Influence of DNA Extraction and Primer Choice on the Observed Microbial Communities in Drinking Water Samples Using 16S rRNA Gene Amplicon Sequencing.Frontiers in microbiology · 2018Article
- Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.BMC microbiology · 2017Article
- Development of a cost-effective metabarcoding strategy for analysis of the marine phytoplankton community.PeerJ · 2016Article
- Bacterial release from pipe biofilm in a full-scale drinking water distribution system.NPJ biofilms and microbiomesArticle
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
Abstract
The applicability of next generation DNA sequencing (NGS) methods for water quality assessment has so far not been broadly investigated. This study set out to evaluate the potential of an NGS-based approach in a complex catchment with importance for drinking water abstraction. In this multi-compartment investigation, total bacterial communities in water, faeces, soil, and sediment samples were investigated by 454 pyrosequencing of bacterial 16S rRNA gene amplicons to assess the capabilities of this NGS method for (i) the development and evaluation of environmental molecular diagnostics, (ii) direct screening of the bulk bacterial communities, and (iii) the detection of faecal pollution in water. Results indicate that NGS methods can highlight potential target populations for diagnostics and will prove useful for the evaluation of existing and the development of novel DNA-based detection methods in the field of water microbiology. The used approach allowed unveiling of dominant bacterial populations but failed to detect populations with low abundances such as faecal indicators in surface waters. In combination with metadata, NGS data will also allow the identification of drivers of bacterial community composition during water treatment and distribution, highlighting the power of this approach for monitoring of bacterial regrowth and contamination in technical systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.