ArticlePloS one2015
Abiotic Stresses Downregulate Key Genes Involved in Nitrogen Uptake and Assimilation in Brassica juncea L.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 167 citations in OpenAlex.
- A balancing act: how plants integrate nitrogen and water signals.Journal of experimental botany · 2020Pooled it
- Process intensification for the protein production from microalgae: a comprehensive review.Bioresources and bioprocessing · 2026Review
- SaNAR2.2, A Novel Member of the NAR2 Family from the EuhalophyteMembranes · 2026Article
- Integrated physiology and multi-omics elucidate photosynthetic adaptation and osmolyte accumulation mechanisms in buffalograss under osmotic stress.BMC plant biology · 2026Article
- Antagonistic regulation of nitrogen and drought signaling mediated by NIN-like protein 7 transcription factor inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nitrogen Use Efficiency in Agriculture: Integrating Biotechnology, Microbiology, and Novel Delivery Systems for Sustainable Agriculture.Plants (Basel, Switzerland) · 2025Review
- High Dose of Nickel Unbalances Carbon Metabolism and Nitrogen Assimilation in Barley (Plants (Basel, Switzerland) · 2025Article
- Plant Nitrogen Assimilation: A Climate Change Perspective.Plants (Basel, Switzerland) · 2025Review
- Regulation of NO-Generating System Activity in Cucumber Root Response to Cold.International journal of molecular sciences · 2025Article
- Impact of Water Stress on Metabolic Intermediates and Regulators in Broccoli Sprouts, and Cellular Defense Potential of Their Extracts.International journal of molecular sciences · 2025Article
- Regulatory network of ammonium and nitrate uptake and utilization in rice.Frontiers in plant science · 2025Review
- Integrated morphological and physiological plasticity of root for improved seedling growth inFrontiers in plant science · 2025Article
- Systematic characterization of Gossypium GLN family genes reveals a potential function of GhGLN1.1a regulates nitrogen use efficiency in cotton.BMC plant biology · 2024Article
- Identification of candidate genes and residues for improving nitrogen use efficiency in the N-sensitive medicinal plant Panax notoginseng.BMC plant biology · 2024Article
- Partial root-zone drying combined with nitrogen treatments mitigates drought responses in rice.Frontiers in plant science · 2024Article
- Regulatory Mechanisms of Plant Growth-Promoting Rhizobacteria and Plant Nutrition against Abiotic Stresses in Brassicaceae Family.Life (Basel, Switzerland) · 2023Review
- Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants.Frontiers in plant science · 2023Review
- Genome-wide investigation and expression profiles of theFrontiers in plant science · 2023Article
- Genome-Wide Identification of AMT2-Type Ammonium Transporters Reveal That CsAMT2.2 and CsAMT2.3 Potentially Regulate NHInternational journal of molecular sciences · 2022Article
- Salt stress decreases seed yield and postpones growth process of canola (Brassica napus L.) by changing nitrogen and carbon characters.Scientific reports · 2022Article
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2 authors at 1 institution in 1 country.
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Abstract
Abiotic stresses such as salinity, drought and extreme temperatures affect nitrogen (N) uptake and assimilation in plants. However, little is known about the regulation of N pathway genes at transcriptional level under abiotic stress conditions in Brassica juncea. In the present work, genes encoding nitrate transporters (NRT), ammonium transporters (AMT), nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH), asparagines synthetase (ASN) were cloned from Brassica juncea L. var. Varuna. The deduced protein sequences were analyzed to predict their subcellular localization, which confirmed localization of all the proteins in their respective cellular organelles. The protein sequences were also subjected to conserved domain identification, which confirmed presence of characteristic domains in all the proteins, indicating their putative functions. Moreover, expression of these genes was studied after 1h and 24h of salt (150 mM NaCl), osmotic (250 mM Mannitol), cold (4°C) and heat (42°C) stresses. Most of the genes encoding nitrate transporters and enzymes responsible for N assimilation and remobilization were found to be downregulated under abiotic stresses. The expression of BjAMT1.2, BjAMT2, BjGS1.1, BjGDH1 and BjASN2 was downregulated after 1hr, while expression of BjNRT1.1, BjNRT2.1, BjNiR1, BjAMT2, BjGDH1 and BjASN2 was downregulated after 24h of all the stress treatments. However, expression of BjNRT1.1, BjNRT1.5 and BjGDH2 was upregulated after 1h of all stress treatments, while no gene was found to be upregulated after 24h of stress treatments, commonly. These observations indicate that expression of most of the genes is adversely affected under abiotic stress conditions, particularly under prolonged stress exposure (24h), which may be one of the reasons of reduction in plant growth and development under abiotic stresses.
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