Evidence map›Paper›PMID 26605798›Full record

ArticlePloS one2015

The Selective Myosin II Inhibitor Blebbistatin Reversibly Eliminates Gastrovascular Flow and Stolon Tip Pulsations in the Colonial Hydroid Podocoryna carnea.

Noah Connally, Christopher P Anderson, Jules E Bolton, Edward W Bolton, Leo W Buss

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Noah ConnallyDepartment of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.
Christopher P AndersonDepartment of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.
Jules E Bolton20 Colony Road, New Haven, Connecticut, United States of America.
Edward W BoltonDepartment of Geology and Geophysics, Yale University, New Haven, Connecticut, United States of America.
Leo W BussDepartment of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Blebbistatin reversibly disrupted both stolon tip pulsations and gastrovascular flow in the colonial hydroid Podocoryna carnea. Epithelial longitudinal muscles of polyps were unaffected by blebbistatin, as polyps contracted when challenged with a pulse of KCl. Latrunculin B, which sequesters G actin preventing F actin assembly, caused stolons to retract, exposing focal adhesions where the tip epithelial cells adhere to the substratum. These results are consistent with earlier suggestions that non-muscle myosin II provides the motive force for stolon tip pulsations and further suggest that tip oscillations are functionally coupled to hydrorhizal axial muscle contraction.

Indexed as

AnimalsHeterocyclic Compounds, 4 or More RingsHydrozoaMuscle ContractionMyosin Type IITime-Lapse ImagingblebbistatinHeterocyclic Compounds, 4 or More RingsMyosin Type II

Identifiers

PMID26605798
PMCPMC4659590

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.