ArticleAnalytical chemistry2016
Nanoparticle-Assisted Removal of Protein in Human Serum for Metabolomics Studies.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Untargeted mass spectrometry to investigate ocean acidification inThe Analyst · 2026Article
- Nanoparticle-assisted strategies in mass spectrometry-based metabolite analysis: recent advances across analytical workflow.Mikrochimica acta · 2025Review
- Surface Modification of Gold Nanoparticle Impacts Distinct Lipid Metabolism.Molecules (Basel, Switzerland) · 2025Article
- COLMAR1d: A Web Server for Automated, Quantitative One-Dimensional Nuclear Magnetic Resonance-Based Metabolomics at Arbitrary Magnetic Fields.Analytical chemistry · 2024Article
- Practical Aspects of NMR-Based Metabolomics.Handbook of experimental pharmacology · 2023Article
- Quantitative plasma profiling byFrontiers in molecular biosciences · 2023Article
- A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers.Drug delivery and translational research · 2022Article
- Nuclear Magnetic Resonance Spectroscopy in Clinical Metabolomics and Personalized Medicine: Current Challenges and Perspectives.Frontiers in molecular biosciences · 2021Review
- Extractive Ratio Analysis NMR Spectroscopy for Metabolite Identification in Complex Biological Mixtures.Analytical chemistry · 2019Article
- An Overview of Metabolic Phenotyping in Blood Pressure Research.Current hypertension reports · 2018Review
- Nanoparticle-Assisted Metabolomics.Metabolites · 2018Review
- Magnetic Nanoparticles: From Design and Synthesis to Real World Applications.Nanomaterials (Basel, Switzerland) · 2017Review
- Knowns and unknowns in metabolomics identified by multidimensional NMR and hybrid MS/NMR methods.Current opinion in biotechnology · 2017Review
- The future of NMR-based metabolomics.Current opinion in biotechnology · 2017Review
- Recent Advances in NMR-Based Metabolomics.Analytical chemistry · 2017Review
- Comprehensive Metabolite Identification Strategy Using Multiple Two-Dimensional NMR Spectra of a Complex Mixture Implemented in the COLMARm Web Server.Analytical chemistry · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Among human body fluids, serum plays a key role for diagnostic tests and, increasingly, for metabolomics analysis. However, the high protein content of serum poses significant challenges for nuclear magnetic resonance (NMR)-based metabolomics studies because it can strongly interfere with metabolite signal detection and quantitation. Although several methods for protein removal have been proposed, including ultrafiltration and organic-solvent-induced protein precipitation, there is currently no standard operating procedure for the elimination of protein from human serum samples. Here, we introduce novel procedures for the removal of protein from serum by the addition of nanoparticles. It is demonstrated how serum protein can be efficiently, cost-effectively, and environmentally friendly removed at physiological pH (pH 7.4) through attractive interactions with silica nanoparticles. It is further shown how serum can be processed with nanoparticles prior to ultrafiltration or organic-solvent-induced protein precipitation for optimal protein removal. After examination of all of the procedures, the combination of nanoparticle treatment and ultrafiltration is found to have a minimal effect on the metabolite content, leading to remarkably clean homo- and heteronuclear NMR spectra of the serum metabolome that compare favorably to other methods for protein removal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.