ArticlePloS one2015
Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Hybrid bioprinting of hierarchical vascular networks at capillary-scale resolution.Nature chemical engineering · 2026Article
- Mass Transfer in Cryopreservation at Multiple Scales: Cells, Tissues, and Organs.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Learning from the kidney: improving heart recovery from hypothermia.Frontiers in cardiovascular medicine · 2026Review
- Microfluidic Platforms for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advances in Cryopreservation Strategies for 3D Biofabricated Constructs: From Hydrogels to Bioprinted Tissues.International journal of molecular sciences · 2025Review
- Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus.Plants (Basel, Switzerland) · 2025Review
- Cell Adhesion and Local Cytokine Control on Protein-Functionalized PNIPAM-co-AAc Hydrogel Microcarriers.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Current State and Challenges of Tissue and Organ Cryopreservation in Biobanking.International journal of molecular sciences · 2024Review
- CPA toxicity screening of cryoprotective solutions in rat hearts.Cryobiology · 2024Article
- Model-Guided Design and Optimization of CPA Perfusion Protocols for Whole Organ Cryopreservation.Annals of biomedical engineering · 2023Article
- Incorporate delivery, warming and washing methods into efficient cryopreservation.Frontiers in bioengineering and biotechnology · 2023Article
- Osmotic response during kidney perfusion with cryoprotectant in isotonic or hypotonic vehicle solution.PeerJ · 2023Article
- Article
- Study of the biological characteristics of human umbilical cord mesenchymal stem cells after long-time cryopreservation.Cell and tissue banking · 2022Article
- Article
- hMSCs in contact with DMSO for cryopreservation: Experiments and modeling of osmotic injury and cytotoxic effect.Biotechnology and bioengineering · 2022Article
- Collagen-Sealed Polyester Vascular Prostheses Functionalized by Polycatecholamine Coatings.International journal of molecular sciences · 2022Article
- Exogenous Melatonin Ameliorates the Negative Effect of Osmotic Stress in Human and Bovine Ovarian Stromal Cells.Antioxidants (Basel, Switzerland) · 2022Article
- Thermal Analyses of Nanowarming-Assisted Recovery of the Heart From Cryopreservation by Vitrification.Journal of heat transfer · 2022Article
- Phytochemical Characterization, Antioxidant Activity, and Cytotoxicity of Methanolic Leaf Extract ofMolecules (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required to suppress ice formation. Recently, we described a mathematical strategy for identifying minimally toxic CPA equilibration procedures based on the minimization of a toxicity cost function. Here we provide direct experimental support for the feasibility of these methods when applied to adherent endothelial cells. We first developed a concentration- and temperature-dependent toxicity cost function by exposing the cells to a range of glycerol concentrations at 21°C and 37°C, and fitting the resulting viability data to a first order cell death model. This cost function was then numerically minimized in our state constrained optimization routine to determine addition and removal procedures for 17 molal (mol/kg water) glycerol solutions. Using these predicted optimal procedures, we obtained 81% recovery after exposure to vitrification solutions, as well as successful vitrification with the relatively slow cooling and warming rates of 50°C/min and 130°C/min. In comparison, conventional multistep CPA equilibration procedures resulted in much lower cell yields of about 10%. Our results demonstrate the potential for rational design of minimally toxic vitrification procedures and pave the way for extension of our optimization approach to other adherent cell types as well as more complex systems such as tissues and organs.
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