Evidence map›Paper›PMID 26605539›Full record

ArticlePloS one2015

West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey.

Christos Hadjichristodoulou, Spyros Pournaras, Maria Mavrouli, Andriani Marka, Persefoni Tserkezou, Agoritsa Baka, Charalambos Billinis, Antonios Katsioulis, Anna Psaroulaki, Anna Papa and 5 more

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Modelling the risk of West Nile virus infection in seven European countries from published serological and case notification data, 2008 to 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2026
    Pooled it
  2. Pooled it
  3. Article
  4. Seroprevalence of West Nile virus, Greece, 2020.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025
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  12. West Nile Virus Seroprevalence in Manisa Province: A Population-based Study.Infectious diseases & clinical microbiology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Christos HadjichristodoulouDepartment of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Spyros PournarasDepartment of Microbiology, Faculty of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Maria MavrouliDepartment of Microbiology, Faculty of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Andriani MarkaDepartment of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Persefoni TserkezouDepartment of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Agoritsa BakaHellenic Centre for Disease Control & Prevention (KEELPNO), Athens, Greece.
Charalambos BillinisLaboratory of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
Antonios KatsioulisDepartment of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Anna PsaroulakiLaboratory of Bacteriology, Parasitology, Zoonoses and Geogrphical Medicine, School of Medicine, University of Crete, Heraklion, Greece.
Anna PapaNational Reference Center for Arboviruses, Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Nikos PapadopoulosLaboratory of Entomology and Agricultural Zoology, School of Agricultural Sciences, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Volos, Greece.
Zissis MamurisDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Athanasios TsakrisDepartment of Microbiology, Faculty of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Jenny KremastinouHellenic Centre for Disease Control & Prevention (KEELPNO), Athens, Greece.
MALWEST Project

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7-2.6) and 1.5% (95% C.I.: 1.2-2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421-1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease.

Indexed as

Population SurveillanceAdolescentAdultAgedAged, 80 and overAntibodies, ViralChildChild, PreschoolCross-Sectional StudiesGeographyGreeceHumansImmunoglobulin GInfantInfant, NewbornMiddle AgedAntibodies, ViralImmunoglobulin G

Identifiers

PMID26605539
PMCPMC4659653

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.