ArticleVirulence2016
Mutant characterization and in vivo conditional repression identify aromatic amino acid biosynthesis to be essential for Aspergillus fumigatus virulence.
Article in Virulence, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- The Aromatic Amino Acid Biosynthesis GeneMicroorganisms · 2025Article
- The Tet-on system for controllable gene expression in the rock-inhabiting black fungus Knufia petricola.Extremophiles : life under extreme conditions · 2024Article
- Characterization ofJournal of medical microbiology · 2024Article
- Genome-scale metabolic modeling of Aspergillus fumigatus strains reveals growth dependencies on the lung microbiome.Nature communications · 2023Article
- Histoplasma capsulatum Relies on Tryptophan Biosynthesis To Proliferate within the Macrophage Phagosome.Infection and immunity · 2023Article
- Expanding the Toolbox for Functional Genomics inJournal of fungi (Basel, Switzerland) · 2023Article
- Lactobacillus rhamnosus colonisation antagonizes Candida albicans by forcing metabolic adaptations that compromise pathogenicity.Nature communications · 2022Article
- Current status and development prospects of aquatic vaccines.Frontiers in immunology · 2022Review
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- The Essential Thioredoxin Reductase of the Human Pathogenic MoldFrontiers in microbiology · 2020Article
- Menacing Mold: Recent Advances in Aspergillus Pathogenesis and Host Defense.Journal of molecular biology · 2019Review
- The Lysine Deacetylase RpdA Is Essential for Virulence inFrontiers in microbiology · 2019Article
- Article
- Standardization ofJournal of fungi (Basel, Switzerland) · 2018Review
- Identification of Antifungal Targets Based on Computer Modeling.Journal of fungi (Basel, Switzerland) · 2018Review
- A Tet-Off gene expression system for validation of antifungal drug targets in a murine invasive pulmonary aspergillosis model.Scientific reports · 2018Article
- Riboflavin and pantothenic acid biosynthesis are crucial for iron homeostasis and virulence in the pathogenic mold Aspergillus fumigatus.Virulence · 2018Article
- Filamentous fungal carbon catabolite repression supports metabolic plasticity and stress responses essential for disease progression.PLoS pathogens · 2017Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenicity of the saprobe Aspergillus fumigatus strictly depends on nutrient acquisition during infection, as fungal growth determines colonisation and invasion of a susceptible host. Primary metabolism has to be considered as a valid target for antimycotic therapy, based on the fact that several fungal anabolic pathways are not conserved in higher eukaryotes. To test whether fungal proliferation during invasive aspergillosis relies on endogenous biosynthesis of aromatic amino acids, defined auxotrophic mutants of A. fumigatus were generated and assessed for their infectious capacities in neutropenic mice and found to be strongly attenuated in virulence. Moreover, essentiality of the complete biosynthetic pathway could be demonstrated, corroborated by conditional gene expression in infected animals and inhibitor studies. This brief report not only validates the aromatic amino acid biosynthesis pathway of A. fumigatus to be a promising antifungal target but furthermore demonstrates feasibility of conditional gene expression in a murine infection model of aspergillosis.
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