Evidence map›Paper›PMID 26605345›Full record

ArticleJournal of immunology research2015

Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy.

Jens Dannull, Chunrui Tan, Christine Farrell, Cynthia Wang, Scott Pruitt, Smita K Nair, Walter T Lee

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Jens DannullDepartment of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Chunrui TanDepartment of Surgery, Division of Otolaryngology-Head and Neck Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Christine FarrellDuke University, Durham, NC 27710, USA.
Cynthia WangDuke University School of Medicine, Durham, NC 27710, USA.
Scott PruittDepartment of Surgery, Duke University Medical Center, Durham, NC 27710, USA ; Division of Experimental Medicine, Merck, Rahway, NJ 07065, USA.
Smita K NairDepartment of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Walter T LeeDepartment of Surgery, Division of Otolaryngology-Head and Neck Surgery, Duke University Medical Center, Durham, NC 27710, USA ; Duke University School of Medicine, Durham, NC 27710, USA ; Durham VA Medical Center, Section of Otolaryngology-Head and Neck Surgery, Durham, NC 27705, USA.
Duke Medical Center · USDuke University · USDuke University Hospital · USMerck & Co., Inc., Rahway, NJ, USA (United States) · US

Funding

PKC Epsilon in Vascular DysfunctionI01BX001398 · VA · VETERANS ADMIN PALO ALTO HEALTH CARE SYS · PI ZHOU, WEI · 2012 to 2015
–
BLRD VA I01 BX001398
6 · The paper itself

Abstract

backgroundDendritic cell- (DC-) tumor fusion cells stimulate effective in vivo antitumor responses. However, therapeutic approaches are dependent upon the coadministration of exogenous 3rd signals. The purpose of this study was to determine the mechanisms for inadequate 3rd signaling by electrofused DC-tumor cell hybrids.

methodsMurine melanoma cells were fused with DCs derived from C57BL/6 mice. Quantitative real-time PCR (qPCR) was used to determine relative changes in Th (T helper) 1 and Th2 cytokine gene expression. In addition, changes in gene expression of fusion cells were determined by microarray. Last, cytokine secretion by fusion cells upon inhibition of signaling pathways was analyzed by ELISA.

resultsqPCR analyses revealed that fusion cells exhibited a downregulation of Th1 associated cytokines IL-12 and IL-15 and an upregulation of the Th2 cytokine IL-4. Microarray studies further showed that the expression of chemokines, costimulatory molecules, and matrix-metalloproteinases was deregulated in fusion cells. Lastly, inhibitor studies demonstrate that inhibition of the PI3K/Akt/mTOR signaling pathway could restore the secretion of bioactive IL-12p70 by fusion cells.

conclusionOur results suggest that combining fusion cell-based vaccination with administration of inhibitors of the PI3K/Akt/mTOR signaling pathway may enhance antitumor responses in patients.

Indexed as

TranscriptomeAnimalsCell FusionCell Line, TumorChemokinesCytokinesDendritic CellsFemaleGene Expression ProfilingHybrid CellsImmunotherapyMatrix MetalloproteinasesMelanoma, ExperimentalMiceNeoplasmsReceptors, ChemokineChemokinesCytokinesMatrix MetalloproteinasesReceptors, ChemokineReceptors, Cytokine

Identifiers

PMID26605345
PMCPMC4641191
OpenAlexW1852753854

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.