Evidence map›Paper›PMID 26604223›Full record

ArticleJournal of cell science2016

Localisation-based imaging of malarial antigens during erythrocyte entry reaffirms a role for AMA1 but not MTRAP in invasion.

David T Riglar, Lachlan Whitehead, Alan F Cowman, Kelly L Rogers, Jake Baum

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

David T RiglarThe Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, 3052, Australia Department of Medical Biology, University of Melbourne, Victoria, 3050, Melbourne, Australia.
Lachlan WhiteheadThe Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, 3052, Australia Department of Medical Biology, University of Melbourne, Victoria, 3050, Melbourne, Australia.
Alan F CowmanThe Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, 3052, Australia Department of Medical Biology, University of Melbourne, Victoria, 3050, Melbourne, Australia.
Kelly L RogersThe Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, 3052, Australia Department of Medical Biology, University of Melbourne, Victoria, 3050, Melbourne, Australia.
Jake BaumDepartment of Life Sciences, Imperial College, London SW7 2AZ, UK jake.baum@imperial.ac.uk.
University of Melbourne · AUImperial College London · GBWalter and Eliza Hall Institute of Medical Research · AU

Funding

Wellcome Trust 100993Wellcome Trust 100993/Z/13/Z
6 · The paper itself

Abstract

Microscopy-based localisation of proteins during malaria parasite (Plasmodium) invasion of the erythrocyte is widely used for tentative assignment of protein function. To date, however, imaging has been limited by the rarity of invasion events and the poor resolution available, given the micron size of the parasite, which leads to a lack of quantitative measures for definitive localisation. Here, using computational image analysis we have attempted to assign relative protein localisation during invasion using wide-field deconvolution microscopy. By incorporating three-dimensional information we present a detailed assessment of known parasite effectors predicted to function during entry but as yet untested or for which data are equivocal. Our method, termed longitudinal intensity profiling, resolves confusion surrounding the localisation of apical membrane antigen 1 (AMA1) at the merozoite-erythrocyte junction and predicts that the merozoite thrombospondin-related anonymous protein (MTRAP) is unlikely to play a direct role in the mechanics of entry, an observation supported with additional biochemical evidence. This approach sets a benchmark for imaging of complex micron-scale events and cautions against simplistic interpretations of small numbers of representative images for the assignment of protein function or prioritisation of candidates as therapeutic targets.

Indexed as

Imaging, Three-DimensionalActinsAntibodies, ProtozoanAntibody SpecificityAntigens, ProtozoanEpitopesErythrocytesFructose-Bisphosphate AldolaseHumansMerozoitesModels, BiologicalPlasmodium falciparumProtein Structure, TertiaryProtein TransportProtozoan ProteinsTight JunctionsActinsAntibodies, ProtozoanAntigens, ProtozoanEpitopesFructose-Bisphosphate AldolaseProtozoan ProteinsDeconvolutionErythrocyte invasionMerozoitePlasmodium falciparumTight junction

Identifiers

PMID26604223
PMCPMC4732298
OpenAlexW2263127316

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.