ArticlePlacenta2016
Changes in the enhancer landscape during early placental development uncover a trophoblast invasion gene-enhancer network.
Article in Placenta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 41 citations in OpenAlex.
- Diminished Maternal Tryptophan Leads to Sexually Dimorphic Differences in the Placenta-Brain Axis.Nutrients · 2026Article
- Review
- Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development.Protein & cell · 2026Article
- Disruption of the Placenta-Brain Axis in Transgenic Mice Lacking Serotonin Transporter (SERT) in Trophoblast Cells.International journal of molecular sciences · 2025Article
- Characterization of a cis-regulatory element upstream of matrix metalloproteinase-9.Scientific reports · 2025Article
- Super-Enhancers in Placental Development and Diseases.Journal of developmental biology · 2025Review
- Placenta Extracellular Vesicles: Messengers Connecting Maternal and Fetal Systems.Biomolecules · 2024Review
- HLA-C expression in extravillous trophoblasts is determined by an ELF3-NLRP2/NLRP7 regulatory axis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Extracellular vesicles from mouse trophoblast cells: Effects on neural progenitor cells and potential participants in the placenta-brain axis†.Biology of reproduction · 2024Article
- Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape.Nature communications · 2023Article
- Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage.Development (Cambridge, England) · 2023Article
- Super-enhancer-associated transcription factors collaboratively regulate trophoblast-active gene expression programs in human trophoblast stem cells.Nucleic acids research · 2023Article
- CORE CONSERVED TRANSCRIPTIONAL REGULATORY NETWORKS DEFINE THE INVASIVE TROPHOBLAST CELL LINEAGE.bioRxiv : the preprint server for biology · 2023Article
- Identifying novel regulators of placental development using time-series transcriptome data.Life science alliance · 2023Article
- Transcriptional regulation ofFrontiers in cell and developmental biology · 2022Article
- Mapping cis-regulatory elements in the midgestation mouse placenta.Scientific reports · 2021Article
- Article
- ELF3 activated by a superenhancer and an autoregulatory feedback loop is required for high-level HLA-C expression on extravillous trophoblasts.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- TLR5 Supports Development of Placental Labyrinthine Zone in Mice.Frontiers in cell and developmental biology · 2021Article
- PlacentaCellEnrich: A tool to characterize gene sets using placenta cell-specific gene enrichment analysis.Placenta · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
introductionTrophoblast invasion establishes adequate blood flow between mother and fetus in early placental development. However, little is known about the cis-regulatory mechanisms underlying this important process. We aimed to identify enhancer elements that are active during trophoblast invasion, and build a trophoblast invasion gene-enhancer network.
methodsWe carried out ChIP-Seq for an enhancer-associated mark (H3k27Ac) at two time points during early placental development in mouse. One time point when invasion is at its peak (e7.5) and another time point shortly afterwards (e9.5). We use computational analysis to identify putative enhancers, as well as the transcription factor binding sites within them, that are specific to the time point of trophoblast invasion.
resultsWe compared read profiles at e7.5 and e9.5 to identify 1,977 e7.5-specific enhancers. Within a subset of e7.5-specific enhancers, we discovered a cell migration associated regulatory code, consisting of three transcription factor motifs: AP1, Ets, and Tcfap2. To validate differential expression of the transcription factors that bind these motifs, we performed RNA-Seq in the same context. Finally, we integrated these data with publicly available protein-protein interaction data and constructed a trophoblast invasion gene-enhancer network. DISCUSSION: The data we generated and analysis we carried out improves our understanding of the regulatory mechanisms of trophoblast invasion, by suggesting a transcriptional code exists in the enhancers of cell migration genes. Furthermore, the network we constructed highlights novel candidate genes that may be critical for trophoblast invasion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.