ArticleNucleic acids research2016
Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 45 citations in OpenAlex.
- Promoter-proximal transcription-translation coupling controls early transcription in Escherichia coli.Nucleic acids research · 2025Article
- Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability.Synthetic and systems biotechnology · 2025Article
- Co-transcriptional gene regulation in eukaryotes and prokaryotes.Nature reviews. Molecular cell biology · 2024Review
- The read-through transcription-mediated autoactivation circuit for virulence regulator expression drives robust type III secretion system 2 expression in Vibrio parahaemolyticus.PLoS pathogens · 2024Article
- Utilizing RNA-seq Data to Infer Bacterial Transcription Termination Sites and Validate Predictions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.RNA biology · 2024Article
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- Identification of a Rho-Dependent Termination SiteMicrobiology spectrum · 2023Article
- Stochastic dynamics and ribosome-RNAP interactions in transcription-translation coupling.Biophysical journal · 2023Article
- Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength.Synthetic and systems biotechnology · 2022Article
- NusG-mediated Coupling of Transcription and Translation Enhances Gene Expression by Suppressing RNA Polymerase Backtracking.Journal of molecular biology · 2022Article
- Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing.Nature communications · 2022Article
- Development and Application of Transcription Terminators for Polyhydroxylkanoates Production in HalophilicFrontiers in microbiology · 2022Article
- Quantitative analysis of asynchronous transcription-translation and transcription processivity iniScience · 2021Article
- Quantitative Control for Stoichiometric Protein Synthesis.Annual review of microbiology · 2021Article
- Clusters of hairpins induce intrinsic transcription termination in bacteria.Scientific reports · 2021Article
- Macromolecular assemblies supporting transcription-translation coupling.Transcription · 2021Review
- A translational riboswitch coordinates nascent transcription-translation coupling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology.
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