ArticleScience advances2015
Kinetics of small molecule interactions with membrane proteins in single cells measured with mechanical amplification.
Article in Science advances, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Quantum relaxometry for detecting biomolecular interactions with single NV centers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quantitative, Label-Free Mapping of Cell Force Dynamics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Photosystem I and ZIF-8 Interfacing: Entrapment and Immobilization.Inorganic chemistry · 2025Article
- Lymphocytes and monocytes undergo swift suppression of IL-10R, IL-6R, and IL-2Rβγ signaling under high concentrations of different cytokines.bioRxiv : the preprint server for biology · 2025Article
- In Situ and Label-Free Quantification of Membrane Protein-Ligand Interactions Using Optical Imaging Techniques: A Review.Biosensors · 2024Review
- Dielectric Surface-Based Biosensors for Enhanced Detection of Biomolecular Interactions: Advances and Applications.Biosensors · 2024Review
- A fluorescence-based binding assay for proteins using the cell surface as a sensing platform.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Electric field modulation of ERK dynamics shows dependency on waveform and timing.Scientific reports · 2024Article
- Ligand Binding-Induced Cellular Membrane Deformation is Correlated with the Changes in Membrane Stiffness.The journal of physical chemistry. B · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Label-free imaging and biomarker analysis of exosomes with plasmonic scattering microscopy.Chemical science · 2022Article
- In Situ Analysis of Membrane-Protein Binding Kinetics and Cell-Surface Adhesion Using Plasmonic Scattering Microscopy.Angewandte Chemie (International ed. in English) · 2022Article
- Label-Free Quantification of Molecular Interaction in Live Red Blood Cells by Tracking Nanometer Scale Membrane Fluctuations.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Critical angle reflection imaging for quantification of molecular interactions on glass surface.Nature communications · 2021Article
- Topological Encoded Vector Beams for Monitoring Amyloid-Lipid Interactions in Microcavity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Optical Imaging of Electrical and Mechanical Couplings between Cells.ACS sensors · 2021Article
- Cell surface thermal proteome profiling tracks perturbations and drug targets on the plasma membrane.Nature methods · 2021Article
- Article
- Simultaneous Quantification of Protein Binding Kinetics in Whole Cells with Surface Plasmon Resonance Imaging and Edge Deformation Tracking.Membranes · 2020Article
- Quantifying Ligand-Protein Binding Kinetics with Self-Assembled Nano-oscillators.Analytical chemistry · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring small molecule interactions with membrane proteins in single cells is critical for understanding many cellular processes and for screening drugs. However, developing such a capability has been a difficult challenge. We show that molecular interactions with membrane proteins induce a mechanical deformation in the cellular membrane, and real-time monitoring of the deformation with subnanometer resolution allows quantitative analysis of small molecule-membrane protein interaction kinetics in single cells. This new strategy provides mechanical amplification of small binding signals, making it possible to detect small molecule interactions with membrane proteins. This capability, together with spatial resolution, also allows the study of the heterogeneous nature of cells by analyzing the interaction kinetics variability between different cells and between different regions of a single cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.