ArticleScience advances2015
Drosophila Dicer-2 has an RNA interference-independent function that modulates Toll immune signaling.
Article in Science advances, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- A dynamic Dif/lncRNA-CR42715/miR-965-3p feedback loop orchestrates toll pathway immunity response in Drosophila.PLoS pathogens · 2026Article
- The integrated stress response suppresses antiviral RNA interference by autophagy-mediated degradation of the RNA-induced silencing complex.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Limited impact of the siRNA pathway on transposable element expression in Aedes aegypti.BMC biology · 2025Article
- In vivo Dicer-2 interactome during viral infection reveals novel pro and antiviral factors in Drosophila melanogaster.PLoS pathogens · 2025Article
- DsRNA as pathogen-associated molecular pattern in innate immunity and multiple functions of the RNAi machinery complicate the use of RNAi in pest control.Frontiers in insect science · 2025Review
- Article
- RNA interference is essential to modulating the pathogenesis of mosquito-borne viruses in the yellow fever mosquitoProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ataxin-2, Twenty-four, and Dicer-2 are components of a noncanonical cytoplasmic polyadenylation complex.Life science alliance · 2022Article
- Apoptotic caspase inhibits innate immune signaling by cleaving NF-κBs in both Mammals and Flies.Cell death & disease · 2022Article
- Characterization of reproductive proteins in the Mexican fruit fly points towards the evolution of novel functions.Proceedings. Biological sciences · 2022Article
- The Aedes aegypti siRNA pathway mediates broad-spectrum defense against human pathogenic viruses and modulates antibacterial and antifungal defenses.PLoS biology · 2022Article
- Enoxacin Shows Broad-Spectrum Antiviral Activity against Diverse Viruses by Enhancing Antiviral RNA Interference in Insects.Journal of virology · 2022Article
- Article
- Minicell-based fungal RNAi delivery for sustainable crop protection.Microbial biotechnology · 2021Article
- Review
- A tissue communication network coordinating innate immune response during muscle stress.Journal of cell science · 2018Article
- Dicer-2 Regulates Resistance and Maintains Homeostasis against Zika Virus Infection inJournal of immunology (Baltimore, Md. : 1950) · 2018Article
- Insect anal droplets contain diverse proteins related to gut homeostasis.BMC genomics · 2018Article
- Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dicer-2 is the central player for small interfering RNA biogenesis in the Drosophila RNA interference (RNAi) pathway. Intriguingly, we found that Dicer-2 has an unconventional RNAi-independent function that positively modulates Toll immune signaling, which defends against Gram-positive bacteria, fungi, and some viruses, in both cells and adult flies. The loss of Dicer-2 expression makes fruit flies more susceptible to fungal infection. We further revealed that Dicer-2 posttranscriptionally modulates Toll signaling because Dicer-2 is required for the proper expression of Toll protein but not for Toll protein stability or Toll mRNA transcription. Moreover, Dicer-2 directly binds to the 3' untranslated region (3'UTR) of Toll mRNA via its PAZ (Piwi/Argonaute/Zwille) domain and is required for protein translation mediated by Toll 3'UTR. The loss of Toll 3'UTR binding activity makes Dicer-2 incapable of promoting Toll signaling. These data indicate that the interaction between Dicer-2 and Toll mRNA plays a pivotal role in Toll immune signaling. In addition, we found that Dicer-2 is also required for the Toll signaling induced by two different RNA viruses in Drosophila cells. Consequently, our findings uncover a novel RNAi-independent function of Dicer-2 in the posttranscriptional regulation of Toll protein expression and signaling, indicate an unexpected intersection of the RNAi pathway and the Toll pathway, and provide new insights into Toll immune signaling, Drosophila Dicer-2, and probably Dicer and Dicer-related proteins in other organisms.
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