ArticleBiomedical optics express2015
Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 42 citations in OpenAlex.
- A coma pattern-based autofocusing method resolves bacterial cold shock response at single-cell level.eLife · 2026Article
- Microscopy with microfluidics in microgravity using FlightScope.NPJ microgravity · 2025Article
- High-Magnification Object Tracking with Ultra-Fast View Adjustment and Continuous Autofocus Based on Dynamic-Range Focal Sweep.Sensors (Basel, Switzerland) · 2024Article
- Three-dimensional fluorescence imaging using the transport of intensity equation.Journal of biomedical optics · 2019Article
- All-optical microscope autofocus based on an electrically tunable lens and a totally internally reflected IR laser.Optics express · 2018Article
- In Vivo Noncontact Imaging of Conjunctival Goblet Cells with Customized Widefield Fluorescence Microscopy.Ophthalmology scienceArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autofocusing is a routine technique in redressing focus drift that occurs in time-lapse microscopic image acquisition. To date, most automatic microscopes are designed on the distance detection scheme to fulfill the autofocusing operation, which may suffer from the low contrast of the reflected signal due to the refractive index mismatch at the water/glass interface. To achieve high autofocusing speed with minimal motion artifacts, we developed a compact multi-band fluorescent microscope with an electrically tunable lens (ETL) device for autofocusing. A modified searching algorithm based on equidistant scanning and curve fitting is proposed, which no longer requires a single-peak focus curve and then efficiently restrains the impact of external disturbance. This technique enables us to achieve an autofocusing time of down to 170 ms and the reproductivity of over 97%. The imaging head of the microscope has dimensions of 12 cm × 12 cm × 6 cm. This portable instrument can easily fit inside standard incubators for real-time imaging of living specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.