Evidence map›Paper›PMID 26601000›Full record

ArticleBiomedical optics express2015

Non-invasive manipulation of Drosophila behavior by two-photon excited red-activatable channelrhodopsin.

Po-Yen Hsiao, Chia-Lun Tsai, Ming-Chang Chen, Yen-Yin Lin, Shang-Da Yang, Ann-Shyn Chiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
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  6. Toward Whole-Body Connectomics.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Po-Yen HsiaoInstitute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
Chia-Lun TsaiInstitute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
Ming-Chang ChenInstitute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yen-Yin LinInstitute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Shang-Da YangInstitute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; shangda@ee.nthu.edu.tw.
Ann-Shyn ChiangInstitute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, CA 92093-0526, USA ; aschiang@life.nthu.edu.tw.
National Tsing Hua University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Scattering and absorption limit light penetration through inhomogeneous tissue. To reduce scattering, biochemists have shifted the wavelengths of excitation light for optogenetic actuators and fluorescent proteins to the orange-red range, while physicists have developed multiphoton technologies for deep tissue stimulation. We have built a rapid multiphoton spectroscopic screening system of genetically encoded red-activatable channelrhodopsin (ReaChR), and considered specific behaviors in transgenic Drosophila melanogaster as readouts to optimize the laser parameters for two-photon optogenetic activation. A wavelength-tunable optical parametric amplifier was adopted as the major light source for widefield two-photon excitation (TPE) of ReaChR. Our assays suggest that the optimized TPE wavelength of ReaChR is 1250 nm. Exploiting its capacity for optogenetic manipulation to induce macroscopic behavioral change, we realized rapid spectroscopic screening of genetically encoded effectors or indicators in vivo, and used modulation of ReaChR in the fly as a successful demonstration of such a system.

Indexed as

(000.1430) Biology and medicine(190.4970) Parametric oscillators and amplifiers(320.7110) Ultrafast nonlinear optics

Identifiers

PMID26601000
PMCPMC4646544
OpenAlexW2179619467

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.