ArticleBiomedical optics express2015
Rapid imaging of surgical breast excisions using direct temporal sampling two photon fluorescent lifetime imaging.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Robust real-time estimation of non-uniform angular velocity and sub-pixel jitter in images captured with resonant scanners.Optics express · 2023Article
- Visualizing the Nucleome Using the CRISPR-Cas9 System: From in vitro to in vivo.Biochemistry. Biokhimiia · 2023Review
- Correction of non-uniform angular velocity and sub-pixel jitter in optical scanning.Optics express · 2022Article
- Dynamic wavefront distortion in resonant scanners.Applied optics · 2021Article
- Single-photon peak event detection (SPEED): a computational method for fast photon counting in fluorescence lifetime imaging microscopy.Optics express · 2021Article
- Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy.Biomedical optics express · 2021Article
- Article
- Real-time video mosaicking to guide handheld in vivo microscopy.Journal of biophotonics · 2020Article
- Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.Journal of biomedical optics · 2020Review
- Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.Nature communications · 2020Article
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- Topical dual-probe staining using quantum dot-labeled antibodies for identifying tumor biomarkers in fresh specimens.PloS one · 2020Article
- Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy.Biomedical optics express · 2019Article
- Real-time detection of breast cancer at the cellular level.Journal of cellular physiology · 2019Review
- Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins.Journal of biomedical optics · 2019Article
- High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy.Optica · 2018Article
- Real-time visualization of two-photon fluorescence lifetime imaging microscopy using a wavelength-tunable femtosecond pulsed laser.Biomedical optics express · 2018Article
- Multiscale nonlinear microscopy and widefield white light imaging enables rapid histological imaging of surgical specimen margins.Biomedical optics express · 2018Article
- Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and translational implications.Translational research : the journal of laboratory and clinical medicine · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Two photon fluorescent lifetime imaging is a modality that enables depth-sectioned, molecularly-specific imaging of cells and tissue using intrinsic contrast. However, clinical applications have not been well explored due to low imaging speed and limited field of view, which make evaluating large pathology samples extremely challenging. To address these limitations, we have developed direct temporal sampling two photon fluorescent lifetime imaging (DTS-FLIM), a method which enables a several order of magnitude increase in imaging speed by capturing an entire lifetime decay in a single fluorescent excitation. We use this greatly increased speed to perform a preliminary study using gigapixel-scale imaging of human breast pathology surgical specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.