ArticleEnzyme research2015
Acetylcholinesterase from Human Erythrocytes as a Surrogate Biomarker of Lead Induced Neurotoxicity.
Article in Enzyme research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- Prediction of chemical-induced acute toxicity using in vitro assay data and chemical structure.Toxicology and applied pharmacology · 2024Article
- Comparison of acetylcholinesterase among employees based on job positions and personal protective equipment in fuel station.Environmental analysis, health and toxicology · 2023Article
- Review
- Ameliorative Impact ofIndian journal of clinical biochemistry : IJCB · 2022Article
- Sublethal effects of chronic exposure to CdO or PbO nanoparticles or their binary mixture on the honey bee (Apis millefera L.).Environmental science and pollution research international · 2020Article
- Positive and inverse correlation of blood lead level with erythrocyte acetylcholinesterase and intelligence quotient in children: implications for neurotoxicity.Interdisciplinary toxicology · 2019Article
- Human Erythrocyte Acetylcholinesterase in Health and Disease.Molecules (Basel, Switzerland) · 2017Review
- Prevalence of chromosomal aberrations in Argentinean agricultural workers.Environmental science and pollution research international · 2017Article
- How and where to perform biomonitoring studies: different levels of toxic metal pollution are detected in the Alagados Reservoir in Southern Brazil.Environmental science and pollution research international · 2017Article
- Synergistic Effects of Heavy Metals and Pesticides in Living Systems.Frontiers in chemistry · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lead induced neurotoxicity in the people engaged in different occupations has received wide attention but very little studies have been carried out to monitor occupational neurotoxicity directly due to lead exposure using biochemical methods. In the present paper an endeavour has been made in order to assess the lead mediated neurotoxicity by in vitro assay of the activity of acetylcholinesterase (AChE) from human erythrocytes in presence of different concentrations of lead. The results suggested that the activity of this enzyme was localized in membrane bound fraction and it was found to be highly stable up to 30 days when stored at -20°C in phosphate buffer (50 mM, pH 7.4) containing 0.2% Triton X-100. The erythrocyte's AChE exhibited K m for acetylcholinesterase to be 0.1 mM. Lead caused sharp inhibition of the enzyme and its IC50 value was computed to be 1.34 mM. The inhibition of the enzyme by lead was found to be of uncompetitive type (K i value, 3.6 mM) which negatively influenced both the V max and the enzyme-substrate binding affinity. Taken together, these results indicate that AChE from human erythrocytes could be exploited as a surrogate biomarker of lead induced neurotoxicity particularly in the people occupationally exposed to lead.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.