ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2015
Isolation of high quality RNA from pistachio (Pistacia vera L.) and other woody plants high in secondary metabolites.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Physiological and molecular responses of two contrasting drought resistance pistachio interspecific hybrid rootstocks.Frontiers in plant science · 2025Article
- Effectiveness of Phosphate and Zinc Solubilizing Paenarthrobacter nitroguajacolicus P1 as Halotolerant Rhizobacterium with Growth-Promoting Activity on Pistacia vera L.Current microbiology · 2023Article
- Response to Cadmium Toxicity: Orchestration of Polyamines and microRNAs in Maize Plant.Plants (Basel, Switzerland) · 2023Article
- Pistachio genomes provide insights into nut tree domestication and ZW sex chromosome evolution.Plant communications · 2023Article
- An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor.Scientific reports · 2022Article
- Peduncle Necking inFrontiers in plant science · 2022Article
- A genome-wide transcriptome map of pistachio (Pistacia vera L.) provides novel insights into salinity-related genes and marker discovery.BMC genomics · 2017Article
- Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The quality and quantity of RNA are critical for successful downstream transcriptome-based studies such as microarrays and RNA sequencing (RNA-Seq). RNA isolation from woody plants, such as Pistacia vera, with very high amounts of polyphenols and polysaccharides is an enormous challenge. Here, we describe a highly efficient protocol that overcomes the limitations posed by poor quality and low yield of isolated RNA from pistachio and various recalcitrant woody plants. The key factors that resulted in a yield of 150 μg of high quality RNA per 200 mg of plant tissue include the elimination of phenol from the extraction buffer, raising the concentration of β-mercaptoethanol, long time incubation at 65 °C, and nucleic acid precipitation with optimized volume of NaCl and isopropyl alcohol. Also, the A260/A280 and A260/A230 of extracted RNA were about 1.9-2.1and 2.2-2.3, respectively, revealing the high purity. Since the isolated RNA passed highly stringent quality control standards for sensitive reactions, including RNA sequencing and real-time PCR, it can be considered as a reliable and cost-effective method for RNA extraction from woody plants.
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Registered trials
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