ArticlePloS one2015
Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy.Patterns (New York, N.Y.) · 2025Review
- Three-dimensional tracking using a single-spot rotating point spread function created by a multiring spiral phase plate.Journal of biomedical optics · 2022Article
- A basal-level activity of ATR links replication fork surveillance and stress response.Molecular cell · 2021Article
- Adaptive optics in super-resolution microscopy.Biophysics reports · 2021Article
- Accurate localization microscopy by intrinsic aberration calibration.Nature communications · 2021Article
- Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.Nature methods · 2020Article
- Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization.Biomedical optics express · 2019Article
- Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.eLife · 2019Article
- Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].Optics express · 2018Review
- A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.Nature communications · 2018Article
- Subnanometer localization accuracy in widefield optical microscopy.Light, science & applications · 2018Article
- Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions.Biomedical optics express · 2017Article
- Measurement-based estimation of global pupil functions in 3D localization microscopy.Optics express · 2017Article
- Analyzing Single Molecule Localization Microscopy Data Using Cubic Splines.Scientific reports · 2017Article
- Navigating challenges in the application of superresolution microscopy.The Journal of cell biology · 2017Review
- Characterisation of the effects of optical aberrations in single molecule techniques.Biomedical optics express · 2016Article
- Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements.Optica · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, which we term `wobble`, results in warped 3D SR images and provide a software tool to correct this distortion. This system-specific, lateral shift is typically > 80 nm across an axial range of ~ 1 μm. A theoretical analysis based on phase retrieval data from our microscope suggests that the wobble is caused by non-rotationally symmetric phase and amplitude aberrations in the microscope's pupil function. We then apply our correction to the bacterial cytoskeletal protein FtsZ in live bacteria and demonstrate that the corrected data more accurately represent the true shape of this vertically-oriented ring-like structure. We also include this correction method in a registration procedure for dual-color, 3D SR data and show that it improves target registration error (TRE) at the axial limits over an imaging depth of 1 μm, yielding TRE values of < 20 nm. This work highlights the importance of correcting aberrations in 3D SR to achieve high fidelity between the measurements and the sample.
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