ArticlePloS one2015
Incidence of Type II CRISPR1-Cas Systems in Enterococcus Is Species-Dependent.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Characterization of the CRISPR1-Cas array and its subtyping potential in Enterococcus faecalis from Malaysia.Access microbiology · 2026Article
- Article
- Relationship Between CRISPR-Cas Systems and Acquisition of Tetracycline Resistance in Non-ClinicalAntibiotics (Basel, Switzerland) · 2025Article
- CRISPR-Cas systems in enterococci.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Review
- CRISPR-Cas inhibits plasmid transfer and immunizes bacteria against antibiotic resistance acquisition in manure.Applied and environmental microbiology · 2024Article
- CRISPR-Cas System, Antimicrobial Resistance, andBiomedicines · 2024Article
- Comparative Analysis of CRISPR-Cas Systems inGenes · 2023Article
- Two Novel Lytic Bacteriophages InfectingViruses · 2022Article
- Genomic analysis ofMolecular biology research communications · 2022Article
- Prevalence of CRISPR-Cas Systems and Their Possible Association with Antibiotic Resistance inInfection and drug resistance · 2022Article
- Let Me Upgrade You: Impact of Mobile Genetic Elements on Enterococcal Adaptation and Evolution.Journal of bacteriology · 2021Review
- Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis.Annals of clinical microbiology and antimicrobials · 2021Article
- Draft Genome Sequence of Enterococcus faecalis AS003, a Strain Possessing All Three Type II-a CRISPR Loci.Microbiology resource announcements · 2021Article
- Molecular Epidemiology ofFrontiers in microbiology · 2020Article
- Enterococcal Genetics.Microbiology spectrum · 2019Review
- Bacterial CRISPR Regions: General Features and their Potential for Epidemiological Molecular Typing Studies.The open microbiology journal · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
CRISPR-Cas systems, which obstruct both viral infection and incorporation of mobile genetic elements by horizontal transfer, are a specific immune response common to prokaryotes. Antiviral protection by CRISPR-Cas comes at a cost, as horizontally-acquired genes may increase fitness and provide rapid adaptation to habitat change. To date, investigations into the prevalence of CRISPR have primarily focused on pathogenic and clinical bacteria, while less is known about CRISPR dynamics in commensal and environmental species. We designed PCR primers and coupled these with DNA sequencing of products to detect and characterize the presence of cas1, a universal CRISPR-associated gene and proxy for the Type II CRISPR1-Cas system, in environmental and non-clinical Enterococcus isolates. CRISPR1-cas1 was detected in approximately 33% of the 275 strains examined, and differences in CRISPR1 carriage between species was significant. Incidence of cas1 in E. hirae was 73%, nearly three times that of E. faecalis (23.6%) and 10 times more frequent than in E. durans (7.1%). Also, this is the first report of CRISPR1 presence in E. durans, as well as in the plant-associated species E. casseliflavus and E. sulfureus. Significant differences in CRISPR1-cas1 incidence among Enterococcus species support the hypothesis that there is a tradeoff between protection and adaptability. The differences in the habitats of enterococcal species may exert varying selective pressure that results in a species-dependent distribution of CRISPR-Cas systems.
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