ArticlePloS one2015
High-Throughput Screening of Myometrial Calcium-Mobilization to Identify Modulators of Uterine Contractility.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Utero-placental calcium and magnesium ion channels: A systematic review of obstetric implications of their alterations.Channels (Austin, Tex.) · 2026Pooled it
- A phenotypicHuman reproduction open · 2026Article
- Myometrial mechanotransduction and the timing of parturition: ion-channel pathways linking uterine stretch, calcium signaling, and inflammation.Frontiers in physiology · 2026Review
- Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix.Science advances · 2024Article
- Transcription Factor 23 is an Essential Determinant of Murine Term Parturition.Molecular and cellular biology · 2024Article
- Arrest of mouse preterm labor until term delivery by combination therapy with atosiban and mundulone, a natural product with tocolytic efficacy.Pharmacological research · 2023Article
- Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity.Current research in physiology · 2023Article
- Effects of Solvents, Emulsions, Cosolvents, and Complexions on Ex Vivo Mouse Myometrial Contractility.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Next generation strategies for preventing preterm birth.Advanced drug delivery reviews · 2021Review
- Tools, techniques, and future opportunities for characterizing the mechanobiology of uterine myometrium.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Study of Magnesium Formulations on Intestinal Cells to Influence Myometrium Cell Relaxation.Nutrients · 2020Article
- Drug discovery strategies for the identification of novel regulators of uterine contractility.Current opinion in physiology · 2020Article
- Comparative analysis of myometrial and vascular smooth muscle cells to determine optimal cells for use in drug discovery.Pharmacological research · 2019Article
- Increased rate of acute caesarean sections in women with epilepsy: results from the Oppland Perinatal Database in Norway.European journal of neurology · 2019Article
- Oxytocin/vasopressin-like peptide inotocin regulates cuticular hydrocarbon synthesis and water balancing in ants.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling.Scientific reports · 2018Article
- Beta-Estradiol Regulates Voltage-Gated Calcium Channels and Estrogen Receptors in Telocytes from Human Myometrium.International journal of molecular sciences · 2018Article
- Effect of molecular hydrogen on uterine inflammation during preterm labour.Biomedical reports · 2018Article
- Monitoring uterine contractility in mice using a transcervical intrauterine pressure catheter.Reproduction (Cambridge, England) · 2018Article
- Elastomeric sensor surfaces for high-throughput single-cell force cytometry.Nature biomedical engineering · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The uterine myometrium (UT-myo) is a therapeutic target for preterm labor, labor induction, and postpartum hemorrhage. Stimulation of intracellular Ca2+-release in UT-myo cells by oxytocin is a final pathway controlling myometrial contractions. The goal of this study was to develop a dual-addition assay for high-throughput screening of small molecular compounds, which could regulate Ca2+-mobilization in UT-myo cells, and hence, myometrial contractions. Primary murine UT-myo cells in 384-well plates were loaded with a Ca2+-sensitive fluorescent probe, and then screened for inducers of Ca2+-mobilization and inhibitors of oxytocin-induced Ca2+-mobilization. The assay exhibited robust screening statistics (Z´ = 0.73), DMSO-tolerance, and was validated for high-throughput screening against 2,727 small molecules from the Spectrum, NIH Clinical I and II collections of well-annotated compounds. The screen revealed a hit-rate of 1.80% for agonist and 1.39% for antagonist compounds. Concentration-dependent responses of hit-compounds demonstrated an EC50 less than 10μM for 21 hit-antagonist compounds, compared to only 7 hit-agonist compounds. Subsequent studies focused on hit-antagonist compounds. Based on the percent inhibition and functional annotation analyses, we selected 4 confirmed hit-antagonist compounds (benzbromarone, dipyridamole, fenoterol hydrobromide and nisoldipine) for further analysis. Using an ex vivo isometric contractility assay, each compound significantly inhibited uterine contractility, at different potencies (IC50). Overall, these results demonstrate for the first time that high-throughput small-molecules screening of myometrial Ca2+-mobilization is an ideal primary approach for discovering modulators of uterine contractility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.