ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2016
Multiparameter analysis of stimulated human peripheral blood mononuclear cells: A comparison of mass and fluorescence cytometry.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 60 citations in OpenAlex.
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- Immunophenotypic predictors of influenza vaccine immunogenicity in pediatric hematopoietic cell transplant recipients.Blood advances · 2024Article
- TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent of CD226 co-stimulation.Nature communications · 2023Article
- Multiparameter analysis of human B lymphocytes identifies heterogeneous CD19Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- OMIP-087: Thirty-two parameter mass cytometry panel to assess human CD4 and CD8 T cell activation, memory subsets, and helper subsets.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- Techniques for Profiling the Cellular Immune Response and Their Implications for Interventional Oncology.Cancers · 2022Review
- Multiplatform Single-Cell Analysis Identifies Immune Cell Types Enhanced in Pulmonary Fibrosis.American journal of respiratory cell and molecular biology · 2022Article
- A roadmap for translational cancer glycoimmunology at single cell resolution.Journal of experimental & clinical cancer research : CR · 2022Review
- CyTOFFrontiers in immunology · 2022Review
- Single-cell analysis shows that adipose tissue of persons with both HIV and diabetes is enriched for clonal, cytotoxic, and CMV-specific CD4+ T cells.Cell reports. Medicine · 2021Article
- Article
- High-Dimensional Data Analysis Algorithms Yield Comparable Results for Mass Cytometry and Spectral Flow Cytometry Data.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Training Novices in Generation and Analysis of High-Dimensional Human Cell Phospho-Flow Cytometry Data.Current protocols in cytometry · 2020Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Lanthanide nanoparticles for high sensitivity multiparameter single cell analysis.Chemical science · 2019Article
- Computational Immune Monitoring Reveals Abnormal Double-Negative T Cells Present across Human Tumor Types.Cancer immunology research · 2019Article
- Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials.Frontiers in oncology · 2019Article
- Tolerating Factor VIII: Recent Progress.Frontiers in immunology · 2019Review
- BRAF and MEK inhibitor therapy eliminates Nestin-expressing melanoma cells in human tumors.Pigment cell & melanoma research · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Mass and fluorescence cytometry are quantitative single cell flow cytometry approaches that are powerful tools for characterizing diverse tissues and cellular systems. Here mass cytometry was directly compared with fluorescence cytometry by studying phenotypes of healthy human peripheral blood mononuclear cells (PBMC) in the context of superantigen stimulation. One mass cytometry panel and five fluorescence cytometry panels were used to measure 20 well-established lymphocyte markers of memory and activation. Comparable frequencies of both common and rare cell subpopulations were observed with fluorescence and mass cytometry using biaxial gating. The unsupervised high-dimensional analysis tool viSNE was then used to analyze data sets generated from both mass and fluorescence cytometry. viSNE analysis effectively characterized PBMC using eight features per cell and identified similar frequencies of activated CD4+ T cells with both technologies. These results suggest combinations of unsupervised analysis programs and extended multiparameter cytometry will be indispensable tools for detecting perturbations in protein expression in both health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.