ArticleStem cell research2015
Characterization of neurons from immortalized dental pulp stem cells for the study of neurogenetic disorders.
Article in Stem cell research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Neural Tissue-Like, not Supraphysiological, Electrical Conductivity Stimulates Neuronal Lineage Specification through Calcium Signaling and Epigenetic Modification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.Nature communications · 2023Article
- Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS.Frontiers in pediatrics · 2023Article
- Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells.Current protocols · 2022Article
- Betaine promotes osteogenic differentiation in immortalized human dental pulp-derived cells.BDJ open · 2022Article
- The potential roles of dental pulp stem cells in peripheral nerve regeneration.Frontiers in neurology · 2022Review
- Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration.International journal of molecular sciences · 2021Review
- Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility.Frontiers in molecular neuroscience · 2021Article
- Proteomic Profiling of the First Human Dental Pulp Mesenchymal Stem/Stromal Cells from Carbonic Anhydrase II Deficiency Osteopetrosis Patients.International journal of molecular sciences · 2020Article
- Neuro-regenerative potential of dental stem cells: a concise review.Cell and tissue research · 2020Review
- N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM.International journal of molecular sciences · 2020Article
- Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production.JCI insight · 2020Article
- Regenerative Capacity of Dental Pulp Stem Cells: A Systematic Review.Journal of pharmacy & bioallied sciences · 2020Review
- The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer.Bioscience reports · 2020Article
- Biocompatibility between Silicon or Silicon Carbide surface and Neural Stem Cells.Scientific reports · 2019Article
- Identification of differential gene expression in endothelial cells from young and aged mice using RNA-Seq technique.American journal of translational research · 2019Article
- Mechanisms underlying dental-derived stem cell-mediated neurorestoration in neurodegenerative disorders.Stem cell research & therapy · 2018Review
- Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons.Molecular autism · 2018Article
- Dental pulp stem cells for the study of neurogenetic disorders.Human molecular genetics · 2017Review
- Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns.Stem cells (Dayton, Ohio) · 2017Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
A major challenge to the study and treatment of neurogenetic syndromes is accessing live neurons for study from affected individuals. Although several sources of stem cells are currently available, acquiring these involve invasive procedures, may be difficult or expensive to generate and are limited in number. Dental pulp stem cells (DPSCs) are multipotent stem cells that reside deep the pulp of shed teeth. To investigate the characteristics of DPSCs that make them a valuable resource for translational research, we performed a set of viability, senescence, immortalization and gene expression studies on control DPSC and derived neurons. We investigated the basic transport conditions and maximum passage number for primary DPSCs. We immortalized control DPSCs using human telomerase reverse transcriptase (hTERT) and evaluated neuronal differentiation potential and global gene expression changes by RNA-seq. We show that neurons from immortalized DPSCs share morphological and electrophysiological properties with non-immortalized DPSCs. We also show that differentiation of DPSCs into neurons significantly alters gene expression for 1305 transcripts. Here we show that these changes in gene expression are concurrent with changes in protein levels of the transcriptional repressor REST/NRSF, which is known to be involved in neuronal differentiation. Immortalization significantly altered the expression of 183 genes after neuronal differentiation, 94 of which also changed during differentiation. Our studies indicate that viable DPSCs can be obtained from teeth stored for ≥72 h, these can then be immortalized and still produce functional neurons for in vitro studies, but that constitutive hTERT immortalization is not be the best approach for long term use of patient derived DPSCs for the study of disease.
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