ArticleProceedings of the National Academy of Sciences of the United States of America2015
ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- The Functions of RAB GTPases at the Golgi Complex.Sub-cellular biochemistry · 2026Review
- LC3 and GABARAP independent autophagy of misfolded procollagen in mouse osteoblasts.Autophagy · 2025Article
- GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis.Molecular cancer · 2025Article
- Molecular mechanism for recognition of the cargo adapter Rab6Life science alliance · 2024Article
- The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes.Molecular biology of the cell · 2024Article
- Rab6-mediated retrograde trafficking from the Golgi: the trouble with tubules.Small GTPases · 2023Review
- Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes.Nature biotechnology · 2023Article
- Diagnosis of Alzheimer's disease: recommendations of the Scientific Department of Cognitive Neurology and Aging of the Brazilian Academy of Neurology.Dementia & neuropsychologia · 2022Article
- Article
- Noncanonical ER-Golgi trafficking and autophagy of endogenous procollagen in osteoblasts.Cellular and molecular life sciences : CMLS · 2021Article
- Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation.Cells · 2021Review
- The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.Molecular biology of the cell · 2021Article
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- Article
- Misfolded GPI-anchored proteins are escorted through the secretory pathway by ER-derived factors.eLife · 2019Article
- BML-265 and Tyrphostin AG1478 Disperse the Golgi Apparatus and Abolish Protein Transport in Human Cells.Frontiers in cell and developmental biology · 2019Article
- GBF1 and Arf1 interact with Miro and regulate mitochondrial positioning within cells.Scientific reports · 2018Article
- Intermediate compartment (IC): from pre-Golgi vacuoles to a semi-autonomous membrane system.Histochemistry and cell biology · 2018Review
- Noncanonical autophagy at ER exit sites regulates procollagen turnover.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Development and Enhancement of FRAP as a Key Tool for Investigating Protein Dynamics.Biophysical journal · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Whether Golgi enzymes remain localized within the Golgi or constitutively cycle through the endoplasmic reticulum (ER) is unclear, yet is important for understanding Golgi dependence on the ER. Here, we demonstrate that the previously reported inefficient ER trapping of Golgi enzymes in a rapamycin-based assay results from an artifact involving an endogenous ER-localized 13-kD FK506 binding protein (FKBP13) competing with the FKBP12-tagged Golgi enzyme for binding to an FKBP-rapamycin binding domain (FRB)-tagged ER trap. When we express an FKBP12-tagged ER trap and FRB-tagged Golgi enzymes, conditions precluding such competition, the Golgi enzymes completely redistribute to the ER upon rapamycin treatment. A photoactivatable FRB-Golgi enzyme, highlighted only in the Golgi, likewise redistributes to the ER. These data establish Golgi enzymes constitutively cycle through the ER. Using our trapping scheme, we identify roles of rab6a and calcium-independent phospholipase A2 (iPLA2) in Golgi enzyme recycling, and show that retrograde transport of Golgi membrane underlies Golgi dispersal during microtubule depolymerization and mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.