ArticleMolecular & cellular proteomics : MCP2016
Multistep Fractionation and Mass Spectrometry Reveal Zwitterionic and Anionic Modifications of the N- and O-glycans of a Marine Snail.
Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Analysis of Caenorhabditis Protein Glycosylation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Species-SpecificMarine drugs · 2023Article
- Negative-mode mass spectrometry in the analysis of invertebrate, fungal, and protist N-glycans.Mass spectrometry reviews · 2022Review
- Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Phosphonate production by marine microbes: Exploring new sources and potential function.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mollusc N-glycosylation: Structures, Functions and Perspectives.Biomolecules · 2021Review
- Glycomics, Glycoproteomics, and Glycogenomics: An Inter-Taxa Evolutionary Perspective.Molecular & cellular proteomics : MCP · 2021Review
- De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs.Biomolecules · 2020Review
- Sulfated and sialylatedThe Journal of biological chemistry · 2020Article
- Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates.Glycoconjugate journal · 2020Review
- Comparisons of N-glycans across invertebrate phyla.Parasitology · 2019Review
- Structures and functions of invertebrate glycosylation.Open biology · 2019Review
- Protein-Specific Analysis of Invertebrate Glycoproteins.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.Molecular & cellular proteomics : MCP · 2018Article
- The parasitic nematode Oesophagostomum dentatum synthesizes unusual glycosaminoglycan-like O-glycans.Glycobiology · 2018Article
- Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannoseThe Journal of biological chemistry · 2018Article
- Core Richness of N-Glycans of Caenorhabditis elegans: A Case Study on Chemical and Enzymatic Release.Analytical chemistry · 2018Article
- Exploring the Glycans of Euglena gracilis.Biology · 2017Article
- The underestimated N-glycomes of lepidopteran species.Biochimica et biophysica acta. General subjects · 2017Article
- Gender and developmental specific N-glycomes of the porcine parasite Oesophagostomum dentatum.Biochimica et biophysica acta. General subjects · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Various studies in the past have revealed that molluscs can produce a wide range of rather complex N-glycan structures, which vary from those occurring in other invertebrate animals; particularly methylated glycans have been found in gastropods, and there are some reports of anionic glycans in bivalves. Due to the high variability in terms of previously described structures and methodologies, it is a major challenge to establish glycomic workflows that yield the maximum amount of detailed structural information from relatively low quantities of sample. In this study, we apply differential release with peptide:N-glycosidases F and A followed by solid-phase extraction on graphitized carbon and reversed-phase materials to examine the glycome of Volvarina rubella (C. B. Adams, 1845), a margin snail of the clade Neogastropoda. The resulting four pools of N-glycans were fractionated on a fused core RP-HPLC column and subject to MALDI-TOF MS and MS/MS in conjunction with chemical and enzymatic treatments. In addition, selected N-glycan fractions, as well as O-glycans released by β-elimination, were analyzed by porous graphitized carbon-LC-MS and MS(n). This comprehensive approach enabled us to determine a number of novel modifications of protein-linked glycans, including N-methyl-2-aminoethylphosphonate on mannose and N-acetylhexosamine residues, core β1,3-linked mannose, zwitterionic moieties on core Galβ1,4Fuc motifs, additional mannose residues on oligomannosidic glycans, and bisubstituted antennal fucose; furthermore, typical invertebrate N-glycans with sulfate and core fucose residues are present in this gastropod.
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