ArticleThe Journal of cell biology2015
Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 172 citations in OpenAlex.
- Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription.Nature communications · 2026Article
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- Inertial sensing of water content in tumor spheroids.Science advances · 2026Article
- Single-cell mass-density measurements using microchannel gradient centrifugation.Scientific reports · 2026Article
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- Tumour Cell Size Control and Its Impact on Tumour Cell Function.Cell proliferation · 2025Review
- High-throughput single-cell density measurements enable dynamic profiling of immune cell and drug response from patient samples.Nature biomedical engineering · 2025Article
- High Molecular Crowding in Repair Foci Surrounding DNA Breaks, Measured by Fluorescence Lifetime Imaging Microscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Cells prioritize the regulation of cell mass density.Science advances · 2025Article
- Label-Free Quantification of Protein Density in Living Cells.Current protocols · 2025Article
- The Role of the Swollen State in Cell Proliferation.The Journal of membrane biology · 2025Review
- Macromolecular Crowding in Cell Stress and Death.Sub-cellular biochemistry · 2025Review
- Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation.Cell reports · 2024Article
- Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cells.Nature structural & molecular biology · 2024Article
- Mechanobiology of the nucleus during the G2-M transition.Nucleus (Austin, Tex.) · 2024Review
- Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Orientation-independent-DIC imaging reveals that a transient rise in depletion attraction contributes to mitotic chromosome condensation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Measurement of protein concentration in bacteria and small organelles under a light transmission microscope.Journal of molecular recognition : JMR · 2024Article
- Hypersensitivity of the vimentin cytoskeleton to net-charge states and Coulomb repulsion.bioRxiv : the preprint server for biology · 2024Article
- Robust trigger wave speed in Xenopus cytoplasmic extracts.Nature communications · 2024Article
25 more citing papers are in PubMed but not listed here.
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6 authors at 2 institutions in 1 country.
Funding
Abstract
Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro-B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.