ArticleNeurobiology of disease2016
Genome-wide alterations in hippocampal 5-hydroxymethylcytosine links plasticity genes to acute stress.
Article in Neurobiology of disease, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Epigenome-wide association studies identify novel DNA methylation sites associated with PTSD: a meta-analysis of 23 military and civilian cohorts.Genome medicine · 2024Pooled it
- Multi-omic data integration improves the resolution of the molecular etiology of autism in a mouse model.Molecular psychiatry · 2026Article
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- Review
- Methylation and expression of glucocorticoid receptor exon-1 variants and FKBP5 in teenage suicide-completers.Translational psychiatry · 2023Article
- A pilot investigation of differential hydroxymethylation levels in patient-derived neural stem cells implicates altered cortical development in bipolar disorder.Frontiers in psychiatry · 2023Article
- Gene by environment interaction mouse model reveals a functional role for 5-hydroxymethylcytosine in neurodevelopmental disorders.Genome research · 2022Article
- Oxidative Dysregulation in Early Life Stress and Posttraumatic Stress Disorder: A Comprehensive Review.Brain sciences · 2021Review
- ZFPM1 Necessary for Development of Serotonergic Projections Related to Anxiety and Contextual Fear Learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- DNA methylation and hydroxymethylation have distinct genome-wide profiles related to axonal regeneration.Epigenetics · 2021Article
- A gene expression atlas for different kinds of stress in the mouse brain.Scientific data · 2020Article
- Genetic aetiology of self-harm ideation and behaviour.Scientific reports · 2020Article
- The Impact of Environmental Factors on 5-Hydroxymethylcytosine in the Brain.Current environmental health reports · 2020Review
- Environmental Exposures, the Epigenome, and African American Women's Health.Journal of urban health : bulletin of the New York Academy of Medicine · 2019Review
- Epigenetic Programming Effects of Early Life Stress: A Dual-Activation Hypothesis.Current genomics · 2018Review
- Differentially Methylated Genes in Saliva are linked to Childhood Stress.Scientific reports · 2018Article
- A multi-dimensional characterization of anxiety in monozygotic twin pairs reveals susceptibility loci in humans.Translational psychiatry · 2017Article
- Article
- Analyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice.Alcohol (Fayetteville, N.Y.) · 2017Article
- New Insights into 5hmC DNA Modification: Generation, Distribution and Function.Frontiers in genetics · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Environmental stress is among the most important contributors to increased susceptibility to develop psychiatric disorders, including anxiety and post-traumatic stress disorder. While even acute stress alters gene expression, the molecular mechanisms underlying these changes remain largely unknown. 5-hydroxymethylcytosine (5hmC) is a novel environmentally sensitive DNA modification that is highly enriched in post-mitotic neurons and is associated with active transcription of neuronal genes. Recently, we found a hippocampal increase of 5hmC in the glucocorticoid receptor gene (Nr3c1) following acute stress, warranting a deeper investigation of stress-related 5hmC levels. Here we used an established chemical labeling and affinity purification method coupled with high-throughput sequencing technology to generate the first genome-wide profile of hippocampal 5hmC following exposure to acute restraint stress and a one-hour recovery. This approach found a genome-wide disruption in 5hmC associated with acute stress response, primarily in genic regions, and identified known and potentially novel stress-related targets that have a significant enrichment for neuronal ontological functions. Integration of these data with hippocampal gene expression data from these same mice found stress-related hydroxymethylation correlated to altered transcript levels and sequence motif predictions indicated that 5hmC may function by mediating transcription factor binding to these transcripts. Together, these data reveal an environmental impact on this newly discovered epigenetic mark in the brain and represent a critical step toward understanding stress-related epigenetic mechanisms that alter gene expression and can lead to the development of psychiatric disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.