ArticleScientific reports2015
Conversion of a light-driven proton pump into a light-gated ion channel.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Driving forces of proton-pumping rhodopsins.Biophysical journal · 2024Article
- Structural characterization of proton-pumping rhodopsin lacking a cytoplasmic proton donor residue by X-ray crystallography.The Journal of biological chemistry · 2022Article
- Article
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Article
- Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.Advances in experimental medicine and biology · 2021Review
- Modified Rhodopsins FromFrontiers in molecular biosciences · 2021Article
- Article
- Multifaceted aspects of charge transfer.Physical chemistry chemical physics : PCCP · 2020Article
- Collective exchange processes reveal an active site proton cage in bacteriorhodopsin.Communications biology · 2020Article
- Engineered Passive Potassium Conductance in the KR2 Sodium Pump.Biophysical journal · 2019Article
- Electrical properties, substrate specificity and optogenetic potential of the engineered light-driven sodium pump eKR2.Scientific reports · 2018Article
- Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae.BMC research notes · 2018Article
- Monod-Wyman-Changeux Analysis of Ligand-Gated Ion Channel Mutants.The journal of physical chemistry. B · 2017Article
- Algal Cell Factories: Approaches, Applications, and Potentials.Marine drugs · 2016Review
- A natural light-driven inward proton pump.Nature communications · 2016Article
- Review
- Structurally Distinct Cation Channelrhodopsins from Cryptophyte Algae.Biophysical journal · 2016Article
- Optogenetic approaches addressing extracellular modulation of neural excitability.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interest in microbial rhodopsins with ion pumping activity has been revitalized in the context of optogenetics, where light-driven ion pumps are used for cell hyperpolarization and voltage sensing. We identified an opsin-encoding gene (CsR) in the genome of the arctic alga Coccomyxa subellipsoidea C-169 that can produce large photocurrents in Xenopus oocytes. We used this property to analyze the function of individual residues in proton pumping. Modification of the highly conserved proton shuttling residue R83 or its interaction partner Y57 strongly reduced pumping power. Moreover, this mutation converted CsR at moderate electrochemical load into an operational proton channel with inward or outward rectification depending on the amino acid substitution. Together with molecular dynamics simulations, these data demonstrate that CsR-R83 and its interacting partner Y57 in conjunction with water molecules forms a proton shuttle that blocks passive proton flux during the dark-state but promotes proton movement uphill upon illumination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.