Evidence map›Paper›PMID 26597546›Full record

ArticleActa biomaterialia2016

Mineral particles modulate osteo-chondrogenic differentiation of embryonic stem cell aggregates.

Yun Wang, Xiaohua Yu, Christopher Baker, William L Murphy, Todd C McDevitt

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Yun WangGladstone Institute of Cardiovascular Disease, The Gladstone Institutes, San Francisco, CA 94158, USA.
Xiaohua YuDepartments of Biomedical Engineering & Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI 53705, USA.
Christopher BakerThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
William L MurphyDepartments of Biomedical Engineering & Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI 53705, USA.
Todd C McDevittGladstone Institute of Cardiovascular Disease, The Gladstone Institutes, San Francisco, CA 94158, USA; Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA. Electronic address: todd.mcdevitt@gladstone.ucsf.edu.
University of Wisconsin–Madison · USGeorgia Institute of Technology · USGladstone Institutes · USUniversity of California, San Francisco · US

Funding

Microsphere-mediated differentiation of embryonic stem cellsR01GM088291 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI MCDEVITT, TODD C · 2009 to 2013
$1.7M
Modulation of the Immune System to Improve Ligament/Ligament Graft HealingR01AR059916 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI MURPHY, WILLIAM L., VANDERBY, RAY · 2010 to 2014
$1.4M
NIAMS NIH HHS R01 AR059916NIAMS NIH HHS R01AR059916NIGMS NIH HHS R01 GM088291NIGMS NIH HHS R01GM088291
6 · The paper itself

Abstract

Pluripotent stem cell aggregates offer an attractive approach to emulate embryonic morphogenesis and skeletal development. Calcium phosphate (CaP) based biomaterials have been shown to promote bone healing due to their osteoconductive and potential osteoinductive properties. In this study, we hypothesized that incorporation of CaP-coated hydroxyapatite mineral particles (MPs) within murine embryonic stem cell (ESC) aggregates could promote osteo-chondrogenic differentiation. Our results demonstrated that MP alone dose-dependently promoted the gene expression of chondrogenic and early osteogenic markers. In combination with soluble osteoinductive cues, MPs enhanced the hypertrophic and osteogenic phenotype, and mineralization of ESC aggregates. Additionally, MPs dose-dependently reduced ESC pluripotency and thereby decreased the size of teratomas derived from MP-incorporated ESC aggregates in vivo. Our data suggested a novel yet simple means of using mineral particles to control stem cell fate and create an osteochondral niche for skeletal tissue engineering applications. STATEMENT OF SIGNIFICANCE: Directing stem cell differentiation and morphogenesis via biomaterials represents a novel strategy to promote cell fates and tissue formation. Our study demonstrates the ability of calcium phosphate-based mineral particles to promote osteochondrogenic differentiation of embryonic stem cell aggregates as well as modulate teratoma formation in vivo. This hybrid biomaterial-ESC aggregate approach serves as an enabling platform to evaluate the ability of biomaterials to regulate stem cell fate and regenerate functional skeletal tissues for clinical applications.

Indexed as

DurapatiteAnimalsCell DifferentiationChondrogenesisMiceMouse Embryonic Stem CellsOsteogenesisDurapatiteDifferentiationEmbryonic stem cellsMineral microparticlesOsteo-chondrogenesis

Identifiers

PMID26597546
PMCPMC4907541
OpenAlexW2191932260

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.