ArticleJournal of experimental botany2016
CHASE domain-containing receptors play an essential role in the cytokinin response of the moss Physcomitrella patens.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 42 citations in OpenAlex.
- A FLOE-related protein regulates the two-dimensional to three-dimensional growth transition in the moss Physcomitrium patens.Development (Cambridge, England) · 2025Article
- Cytokinin and ALOG proteins regulate pluripotent stem cell identity in the mossScience advances · 2024Article
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- The cytokinin receptor OHK4/OsHK4 regulates inflorescence architecture in rice via an IDEAL PLANT ARCHITECTURE1/WEALTHY FARMER'S PANICLE-mediated positive feedback circuit.The Plant cell · 2023Article
- The origin and early evolution of cytokinin signaling.Frontiers in plant science · 2023Review
- How was apical growth regulated in the ancestral land plant? Insights from the development of non-seed plants.Plant physiology · 2022Review
- Article
- Division site determination during asymmetric cell division in plants.The Plant cell · 2022Review
- Cytokinin-CLAVATA cross-talk is an ancient mechanism regulating shoot meristem homeostasis in land plants.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Red Light and Glucose Enhance Cytokinin-Mediated Bud Initial Formation inPlants (Basel, Switzerland) · 2022Article
- Cytokinin Perception in Ancient Plants beyond Angiospermae.International journal of molecular sciences · 2021Article
- Phytohormone biosynthesis and signaling pathways of mosses.Plant molecular biology · 2021Review
- Fundamental mechanisms of the stem cell regulation in land plants: lesson from shoot apical cells in bryophytes.Plant molecular biology · 2021Review
- RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells.The Plant cell · 2018Article
- Light-regulated PAS-containing histidine kinases delay gametophore formation in the moss Physcomitrella patens.Journal of experimental botany · 2018Article
- The effects of repeated whole genome duplication events on the evolution of cytokinin signaling pathway.BMC evolutionary biology · 2018Article
- CHASE-Containing Histidine Kinase Receptors in Apple Tree: From a Common Receptor Structure to Divergent Cytokinin Binding Properties and Specific Functions.Frontiers in plant science · 2017Article
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the molecular basis for cytokinin action is quite well understood in flowering plants, little is known about the cytokinin signal transduction in early diverging land plants. The genome of the bryophyte Physcomitrella patens (Hedw.) B.S. encodes three classical cytokinin receptors, the CHASE domain-containing histidine kinases, CHK1, CHK2, and CHK3. In a complementation assay with protoplasts of receptor-deficient Arabidopsis thaliana as well as in cytokinin binding assays, we found evidence that CHK1 and CHK2 receptors can function in cytokinin perception. Using gene targeting, we generated a collection of CHK knockout mutants comprising single (Δchk1, Δchk2, Δchk3), double (Δchk1,2, Δchk1,3, Δchk2,3), and triple (Δchk1,2,3) mutants. Mutants were characterized for their cytokinin response and differentiation capacities. While the wild type did not grow on high doses of cytokinin (1 µM benzyladenine), the Δchk1,2,3 mutant exhibited normal protonema growth. Bud induction assays showed that all three cytokinin receptors contribute to the triggering of budding, albeit to different extents. Furthermore, while the triple mutant showed no response in this bioassay, the remaining mutants displayed budding responses in a diverse manner to different types and concentrations of cytokinins. Determination of cytokinin levels in mutants showed no drastic changes for any of the cytokinins; thus, in contrast to Arabidopsis, revealing only small impacts of cytokinin signaling on homeostasis. In summary, our study provides a first insight into the molecular action of cytokinin in an early diverging land plant and demonstrates that CHK receptors play an essential role in bud induction and gametophore development.
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