Evidence map›Paper›PMID 26595646›Full record

ArticleJournal of cellular physiology2016

Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis.

Li-An Wu, Feng Wang, Kevin J Donly, Andrew Baker, Chunyan Wan, Daoshu Luo, Mary MacDougall, Shuo Chen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. The role of WNT1 mutant variant (WNT1Annals of human genetics · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Li-An WuDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
Feng WangDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
Kevin J DonlyDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
Andrew BakerDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
Chunyan WanDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
Daoshu LuoDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
Mary MacDougallDepartment of Oral/Maxillofacial Surgery, University of Alabama at Birmingham, School of Dentistry, Birmingham, Alabama.
Shuo ChenDepartment of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, Texas.
The University of Texas Health Science Center at San Antonio · USFujian Medical University · CNUniversity of Alabama at Birmingham · US

Funding

DSPP signaling in dentinogenesisR01DE019802 · NIDCR · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Shuo Chen · 2009 to 2026
$3.4M
Development of iPS cells to study craniometaphyseal dysplasia in humansR21DE019892 · NIDCR · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI LICHTLER, ALEXANDER C, REICHENBERGER, ERNST J · 2009 to 2010
$356k
NIDCR NIH HHS DE19892NIDCR NIH HHS R01 DE019802NIDCR NIH HHS R21 DE019892
6 · The paper itself

Abstract

Bone morphogenetic proteins 2 and 4 (BMP2/4) are essential for osteoblast differentiation and osteogenesis. Generation of a BMP2/4 dual knock-out ((ko/ko)) osteoblastic cell line is a valuable asset for studying effects of BMP2/4 on skeletal development. In this study, our goal was to create immortalized mouse deleted BMP2/4 osteoblasts by infecting adenoviruses with Cre recombinase and green fluorescent protein genes into immortalized murine floxed BMP2/4 osteoblasts. Transduced BMP2/4(ko/ko) cells were verified by green immunofluorescence and PCR. BMP2/4(ko/ko) osteoblasts exhibited small size, slow cell proliferation rate and cell growth was arrested in G1 and G2 phases. Expression of bone-relate genes was reduced in the BMP2/4(ko/ko) cells, resulting in delay of cell differentiation and mineralization. Importantly, extracellular matrix remodeling was impaired in the BMP2/4(ko/ko) osteoblasts as reflected by decreased Mmp-2 and Mmp-9 expressions. Cell differentiation and mineralization were rescued by exogenous BMP2 and/or BMP4. Therefore, we for the first time described establishment of an immortalized deleted BMP2/4 osteoblast line useful for study of mechanisms in regulating osteoblast lineages.

Indexed as

Cell DifferentiationCell ProliferationGene Knockdown TechniquesOsteogenesisAnimalsBone Morphogenetic Protein 2Bone Morphogenetic Protein 4Cell LineCell SizeExtracellular MatrixG1 Phase Cell Cycle CheckpointsG2 Phase Cell Cycle CheckpointsGene Expression RegulationGreen Fluorescent ProteinsIntegrasesMatrix Metalloproteinase 2Bmp2 protein, mouseBmp4 protein, mouseBone Morphogenetic Protein 2Bone Morphogenetic Protein 4Cre recombinaseGreen Fluorescent ProteinsIntegrasesMatrix Metalloproteinase 2Matrix Metalloproteinase 9Mmp2 protein, mouseMmp9 protein, mouse

Identifiers

PMID26595646
PMCPMC4784166
OpenAlexW2173192163

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.