ArticleFrontiers in neural circuits2015
Bulk regional viral injection in neonatal mice enables structural and functional interrogation of defined neuronal populations throughout targeted brain areas.
Article in Frontiers in neural circuits, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Immature olfactory sensory neurons provide complementary input in the intact olfactory system.iScience · 2026Article
- Activity-dependent development of vocal circuits in the neonatal rodent forebrain.EMBO reports · 2026Article
- Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors.iScience · 2025Article
- Impacts of CACNB4 overexpression on dendritic spine density in both sexes and relevance to schizophrenia.Translational psychiatry · 2024Article
- A low-cost device for cryoanesthesia of neonatal rodents.HardwareX · 2023Article
- Targeted approaches to delineate neuronal morphology during early development.Frontiers in cellular neuroscience · 2023Review
- Synapsin II Directly Suppresses Epileptic Seizures In Vivo.Brain sciences · 2022Article
- All-or-none disconnection of pyramidal inputs onto parvalbumin-positive interneurons gates ocular dominance plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A device for stereotaxic viral delivery into the brains of neonatal mice.BioTechniques · 2020Article
- CCKergic Tufted Cells Differentially Drive Two Anatomically Segregated Inhibitory Circuits in the Mouse Olfactory Bulb.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- An inhalation anaesthesia approach for neonatal mice allowing streamlined stereotactic injection in the brain.Journal of neuroscience methods · 2020Article
- Sex differences in dendritic spine density and morphology in auditory and visual cortices in adolescence and adulthood.Scientific reports · 2020Article
- Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains.Journal of visualized experiments : JoVE · 2018Article
- Multiphoton Intravital Calcium Imaging.Current protocols in cytometry · 2018Article
- Cholecystokinin selectively activates short axon cells to enhance inhibition of olfactory bulb output neurons.The Journal of physiology · 2018Article
- A Versatile Method for Viral Transfection of Calcium Indicators in the Neonatal Mouse Brain.Frontiers in neural circuits · 2018Article
- Olfactory Bulb Deep Short-Axon Cells Mediate Widespread Inhibition of Tufted Cell Apical Dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
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3 authors.
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Abstract
The ability to label and manipulate specific cell types is central to understanding the structure and function of neuronal circuits. Here, we have developed a simple, affordable strategy for labeling of genetically defined populations of neurons throughout a targeted brain region: Bulk Regional Viral Injection (BReVI). Our strategy involves a large volume adeno-associated virus (AAV) injection in the targeted brain region of neonatal Cre driver mice. Using the mouse olfactory bulb (OB) as a model system, we tested the ability of BReVI to broadly and selectively label tufted cells, one of the two principal neuron populations of the OB, in CCK-IRES-Cre mice. BReVI resulted in labeling of neurons throughout the injected OB, with no spatial bias toward the injection site and no evidence of damage. The specificity of BReVI labeling was strikingly similar to that seen previously using immunohistochemical staining for cholecystokinin (CCK), an established tufted cell marker. Hence, the CCK-IRES-Cre line in combination with BReVI can provide an important tool for targeting and manipulation of OB tufted cells. We also found robust Cre-dependent reporter expression within three days of BReVI, which enabled us to assess developmental changes in the number and laminar distribution of OB tufted cells during the first three postnatal weeks. Furthermore, we demonstrate that BReVI permits structural and functional imaging in vivo, and can be combined with transgenic strategies to facilitate multi-color labeling of neuronal circuit components. BReVI is broadly applicable to different Cre driver lines and can be used to regionally manipulate genetically defined populations of neurons in any accessible brain region.
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