ArticleAging cell2016
DNA methylation age is associated with mortality in a longitudinal Danish twin study.
Article in Aging cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 189 papers, 7 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Reversing Epigenetic & Other Markers of Senescence by Transfusing Young Plasma To Older Human Subjects
Effects of Tildrakizumab (ILUMYA) Use on Epigenetic Age Acceleration and Psoriasis
Who cites it
189 citing papers in PubMed, 7 syntheses or guidelines pooled it.
- PTSD and epigenetic aging: a longitudinal meta-analysis.Psychological medicine · 2025Pooled it
- Age-associated changes in cumulus cells and follicular fluid: the local oocyte microenvironment as a determinant of gamete quality.Biology of reproduction · 2022Pooled it
- DNA methylation aging and transcriptomic studies in horses.Nature communications · 2022Pooled it
- A systematic review of childhood maltreatment and DNA methylation: candidate gene and epigenome-wide approaches.Translational psychiatry · 2021Pooled it
- The epigenetic clock as a predictor of disease and mortality risk: a systematic review and meta-analysis.Clinical epigenetics · 2019Pooled it
- Traumatic stress and accelerated DNA methylation age: A meta-analysis.Psychoneuroendocrinology · 2018Pooled it
- DNA methylation-based measures of biological age: meta-analysis predicting time to death.Aging · 2016Pooled it
- Nucleotides as an Anti-Aging Supplementation in Older Adults: A Randomized Controlled Trial (TALENTs study).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Trial
- The effect of polyphenols on DNA methylation-assessed biological age attenuation: the DIRECT PLUS randomized controlled trial.BMC medicine · 2023Trial
- Lifestyle weight-loss intervention may attenuate methylation aging: the CENTRAL MRI randomized controlled trial.Clinical epigenetics · 2021Trial
- Change in the Rate of Biological Aging in Response to Caloric Restriction: CALERIE Biobank Analysis.The journals of gerontology. Series A, Biological sciences and medical sciences · 2017Trial
- Epigenome-wide Association of DNA Methylation in Whole Blood With Bone Mineral Density.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Trial
- DNA methylome analysis identifies accelerated epigenetic ageing associated with postmenopausal breast cancer susceptibility.European journal of cancer (Oxford, England : 1990) · 2017Trial
- Late-onset preeclampsia is characterized by accelerated placental aging.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Population epigenetics: deciphering DNA methylation diversity and its implications for health, disease, and evolution.Molecular biology and evolution · 2026Review
- Analysis of second-generation epigenetic clocks reveals further associations between disproportionate biological ageing and hippocampal volume.GeroScience · 2026Article
- Position effect variegation (PEV) as an aging clock: visualization of age-dependent loss of heterochromatin and longevity associated with enhanced heterochromatin.Scientific reports · 2026Article
- Placental epigenetic clocks derived from crowdsourcing: Implications for the study of accelerated aging in obstetrics.iScience · 2025Article
- No indications of weight gain associated DNA methylation changes in patients with anorexia nervosa.Scientific reports · 2025Article
- Estimating Polar Bear (Ecology and evolution · 2025Article
129 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An epigenetic profile defining the DNA methylation age (DNAm age) of an individual has been suggested to be a biomarker of aging, and thus possibly providing a tool for assessment of health and mortality. In this study, we estimated the DNAm age of 378 Danish twins, age 30-82 years, and furthermore included a 10-year longitudinal study of the 86 oldest-old twins (mean age of 86.1 at follow-up), which subsequently were followed for mortality for 8 years. We found that the DNAm age is highly correlated with chronological age across all age groups (r = 0.97), but that the rate of change of DNAm age decreases with age. The results may in part be explained by selective mortality of those with a high DNAm age. This hypothesis was supported by a classical survival analysis showing a 35% (4-77%) increased mortality risk for each 5-year increase in the DNAm age vs. chronological age. Furthermore, the intrapair twin analysis revealed a more-than-double mortality risk for the DNAm oldest twin compared to the co-twin and a 'dose-response pattern' with the odds of dying first increasing 3.2 (1.05-10.1) times per 5-year DNAm age difference within twin pairs, thus showing a stronger association of DNAm age with mortality in the oldest-old when controlling for familial factors. In conclusion, our results support that DNAm age qualifies as a biomarker of aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.