ArticleScientific reports2015
Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Mitochondria regulate inositol triphosphate-mediated CaThe Journal of physiology · 2025Article
- Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard.Developmental cell · 2024Article
- Acute glucose influx-induced mitochondrial hyperpolarization inactivates myosin phosphatase as a novel mechanism of vascular smooth muscle contraction.Cell death & disease · 2021Article
- Mitochondrial Safeguard: a stress response that offsets extreme fusion and protects respiratory function via flickering-induced Oma1 activation.The EMBO journal · 2020Article
- The last wall of defense to prevent extreme and deleterious mitochondrial fusion.The EMBO journal · 2020Article
- Optogenetic control of mitochondrial protonmotive force to impact cellular stress resistance.EMBO reports · 2020Article
- Hydrogen peroxide depolarizes mitochondria and inhibits IPCell calcium · 2019Article
- Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate-evoked calcium signaling.The Journal of biological chemistry · 2019Article
- Spatially structured cell populations process multiple sensory signals in parallel in intact vascular endothelium.Science signaling · 2018Article
- Two-photon deep-tissue spatially resolved mitochondrial imaging using membrane potential fluorescence fluctuations.Biomedical optics express · 2018Article
- Impaired Mitochondrial Respiration in Large Cerebral Arteries of Rats with Type 2 Diabetes.Journal of vascular research · 2017Article
- Acetylcholine released by endothelial cells facilitates flow-mediated dilatation.The Journal of physiology · 2016Article
- Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle.The Journal of physiology · 2016Article
- Role of Mitochondria in Cerebral Vascular Function: Energy Production, Cellular Protection, and Regulation of Vascular Tone.Comprehensive Physiology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Mitochondrial morphology is central to normal physiology and disease development. However, in many live cells and tissues, complex mitochondrial structures exist and morphology has been difficult to quantify. We have measured the shape of electrically-discrete mitochondria, imaging them individually to restore detail hidden in clusters and demarcate functional boundaries. Stochastic "flickers" of mitochondrial membrane potential were visualized with a rapidly-partitioning fluorophore and the pixel-by-pixel covariance of spatio-temporal fluorescence changes analyzed. This Flicker-assisted Localization Microscopy (FaLM) requires only an epifluorescence microscope and sensitive camera. In vascular myocytes, the apparent variation in mitochondrial size was partly explained by densely-packed small mitochondria. In normotensive animals, mitochondria were small spheres or rods. In hypertension, mitochondria were larger, occupied more of the cell volume and were more densely clustered. FaLM provides a convenient tool for increased discrimination of mitochondrial architecture and has revealed mitochondrial alterations that may contribute to hypertension.
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Registered trials
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