Evidence map›Paper›PMID 26592810›Full record

ArticleClinical oral investigations2016

Characterization of A. actinomycetemcomitans strains in subgingival samples from periodontitis subjects in Morocco.

M Mínguez, O K Ennibi, X Pousa, L Lakhdar, L Abdellaoui, M Sánchez, M Sanz, D Herrera

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Article in Clinical oral investigations, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Aggregatibacter actinomycetemcomitans: From Basic to Advanced Research.Advances in experimental medicine and biology · 2022
    Article
  3. CD18 Mediates Neutrophil Imperviousness to theFrontiers in immunology · 2022
    Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

M MínguezSection of Periodontology, Faculty of Odontology, University Complutense, Plaza Ramón y Cajal S/N, 28040, Madrid, Spain.
O K EnnibiClinical Department of Periodontology, Dental School, Mohammed V Souissi University, Rabat, Morocco.
X PousaSection of Periodontology, Faculty of Odontology, University Complutense, Plaza Ramón y Cajal S/N, 28040, Madrid, Spain.
L LakhdarClinical Department of Periodontology, Dental School, Mohammed V Souissi University, Rabat, Morocco.
L AbdellaouiClinical Department of Periodontology, Dental School, Mohammed V Souissi University, Rabat, Morocco.
M SánchezLaboratory of Research, Faculty of Odontology, University Complutense, Madrid, Spain.
M SanzSection of Periodontology, Faculty of Odontology, University Complutense, Plaza Ramón y Cajal S/N, 28040, Madrid, Spain.
D HerreraSection of Periodontology, Faculty of Odontology, University Complutense, Plaza Ramón y Cajal S/N, 28040, Madrid, Spain. davidher@odon.ucm.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAggregatibacter actinomycetemcomitans, specially its highly leucotoxic strain (JP2 clone), represents an etiological factor for the onset and progression of aggressive types of periodontitis. The aims of this investigation were to investigate the most relevant periodontal pathogens in the subgingival microbiota of periodontitis patients from Morocco and to describe the clinical and microbiological characteristics of subjects positive for A. actinomycetemcomitans, including serotype, leukotoxin gene, and operon of the cytolethal distending toxin (cdt) distribution. MATERIAL AND

methodsIn consecutive Moroccan subjects diagnosed of periodontitis, subgingival samples were taken and processed by culture. From the positive samples for A. actinomycetemcomitans, one to three isolates were subcultured and characterized by means of polymerase chain reaction (PCR), assessing their specific serotype distribution, the variation in the sequences of the leukotoxin gene, and the operon of the cdt.

resultsTwenty-one (35.6 %) out of 59 periodontitis patients harbored A. actinomycetemcomitans. These patients demonstrated statistically significant deeper pockets (p = 0.035) and higher proportions of P. micra (p = 0.045) than did the negative group. The 39 studied isolates were serotype "b"; in 16 out of 17 patients, there was mono-colonization with this serotype. Five isolates, from two patients, presented the 530-bp deletion in the leukotoxin's promoter region. Thirty-two isolates (78 % of the strains) were cdt-positive.

conclusionA. actinomycetemcomitans was frequently found (35.6 %) in our sample. All strains were serotype "b," and most (78 %) were also cdt-positive. The JP2 strain type was only detected in 12.2 % of the strains. CLINICAL RELEVANCE: A. actinomycetemcomitans can be frequently found in Morocco. This fact can influence the therapeutic approach of this type of patients.

Indexed as

AdolescentAdultAggregatibacter actinomycetemcomitansChildFemaleHumansMaleMicrobiotaMiddle AgedMoroccoPeriodontitisPolymerase Chain ReactionAggregatibacter actinomycetemcomitansMoroccoPeriodontitisPolymerase chain reactionSubgingival microbiota

Identifiers

PMID26592810

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