ArticleNature communications2015
Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Structural investigation of QatB and QatC proteins in QatABCD anti-phage defense.Nature communications · 2026Article
- Crystal structure of plant γ-glutamyl peptidase 1: implications for sulfur metabolism and secondary metabolite biosynthesis.The FEBS journal · 2026Article
- Crystal structure of guanosine 5'-monophosphate synthetase from the thermophilic bacterium Thermus thermophilus HB8.Acta crystallographica. Section F, Structural biology communications · 2024Article
- GMP Synthetase: Allostery, Structure, and Function.Biomolecules · 2023Review
- Distinct evolution of type I glutamine synthetase in Plasmodium and its species-specific requirement.Nature communications · 2023Article
- Dynamics of the secreted frizzled related protein Sizzled and potential implications for binding to bone morphogenetic protein-1 (BMP-1).Scientific reports · 2022Article
- Tertiary and Quaternary Structure Organization in GMP Synthetases: Implications for Catalysis.Biomolecules · 2022Article
- Mapping of CaPP2C35 involved in the formation of light-green immature pepper (Capsicum annuum L.) fruits via GWAS and BSA.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Review
- Discovery of Novel GMPS Inhibitors ofBiology · 2021Article
- Potential repurposing of four FDA approved compounds with antiplasmodial activity identified through proteome scale computational drug discovery and in vitro assay.Scientific reports · 2021Article
- Role of allosteric switches and adaptor domains in long-distance cross-talk and transient tunnel formation.Science advances · 2020Article
- High temperatures affect the hypersensitive reaction, disease resistance and gene expression induced by a novel harpin HpaG-Xcm.Scientific reports · 2019Article
- Inverse docking based screening and identification of protein targets for Cassiarin alkaloids againstSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2018Article
- GMP Synthase Is Required for Virulence Factor Production and Infection byThe Journal of biological chemistry · 2017Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GMP synthetase (GMPS), a key enzyme in the purine biosynthetic pathway performs catalysis through a coordinated process across two catalytic pockets for which the mechanism remains unclear. Crystal structures of Plasmodium falciparum GMPS in conjunction with mutational and enzyme kinetic studies reported here provide evidence that an 85° rotation of the GATase domain is required for ammonia channelling and thus for the catalytic activity of this two-domain enzyme. We suggest that conformational changes in helix 371-375 holding catalytic residues and in loop 376-401 along the rotation trajectory trigger the different steps of catalysis, and establish the central role of Glu374 in allostery and inter-domain crosstalk. These studies reveal the mechanism of domain rotation and inter-domain communication, providing a molecular framework for the function of all single polypeptide GMPSs and form a solid basis for rational drug design targeting this therapeutically important enzyme.
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