Evidence map›Paper›PMID 26592513›Full record

ArticleActa pharmacologica Sinica2015

Regulation of PP2Cm expression by miRNA-204/211 and miRNA-22 in mouse and human cells.

Bang-fen Pan, Chen Gao, Shu-xun Ren, Yi-bin Wang, Hai-peng Sun, Mei-yi Zhou

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta pharmacologica Sinica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
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  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Bang-fen PanDepartment of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Chen GaoDivision of Molecular Medicine, Department of Anesthesiology, Medicine and Physiology, Molecular Biology Institute, Cardiovascular Research Laboratories, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA.
Shu-xun RenDivision of Molecular Medicine, Department of Anesthesiology, Medicine and Physiology, Molecular Biology Institute, Cardiovascular Research Laboratories, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA.
Yi-bin WangDepartment of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Hai-peng SunDepartment of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Mei-yi ZhouDepartment of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
University of California, Los Angeles · USShanghai Jiao Tong University · CN

Funding

ROLE OF P38 MAPK AND PP2C IN ISCHEMIC INJURY AND PROTECTIONP01HL080111 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, JAMES N · 2005 to 2009
$11.6M
Novel Signaling Mechanisms and Molecular Targets in the Stressed MyocardiumR01HL098954 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PING, PEIPEI, WANG, YIBIN · 2010 to 2013
$3.0M
Novel Mechanism of SR Calcium Regulation in Cardiac DysfunctionR01HL108186 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WANG, YIBIN · 2011 to 2015
$1.9M
Novel Function and Regulatory Mechanisms of Stress Kinase p38 in HeartR01HL103205 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WANG, YIBIN · 2010 to 2014
$1.9M
NHLBI NIH HHS HL080111NHLBI NIH HHS HL098954NHLBI NIH HHS HL103205NHLBI NIH HHS HL108186NHLBI NIH HHS P01 HL080111NHLBI NIH HHS R01 HL098954NHLBI NIH HHS R01 HL103205NHLBI NIH HHS R01 HL108186
6 · The paper itself

Abstract

aimThe mitochondrial targeted 2C-type serine/threonine protein phosphatase (PP2Cm) is encoded by the gene PPM1K and is highly conserved among vertebrates. PP2Cm plays a critical role in branched-chain amino acid catabolism and regulates cell survival. Its expression is dynamically regulated by the nutrient environment and pathological stresses. However, little is known about the molecular mechanism underlying the regulation of PPM1K gene expression. In this study, we aimed to reveal how PPM1K expression is affected by miRNA-mediated post-transcriptional regulation.

methodsComputational analysis based on conserved miRNA binding motifs was applied to predict the candidate miRNAs that potentially affect PPM1K expression. Dual-luciferase reporter assay was performed to verify the miRNAs' binding sites in the PPM1K gene and their influence on PPM1K 3'UTR activity. We further over-expressed the mimics of these miRNAs in human and mouse cells to examine whether miRNAs affected the mRNA level of PPM1K.

resultsComputational analysis identified numerous miRNAs potentially targeting PPM1K. Luciferase reporter assays demonstrated that the 3'UTR of PPM1K gene contained the recognition sites of miR-204 and miR-211. Overexpression of these miRNAs in human and mouse cells diminished the 3'UTR activity and the endogenous mRNA level of PPM1K. However, the miR-22 binding site was found only in human and not mouse PPM1K 3'UTR. Accordingly, PPM1K 3'UTR activity was suppressed by miR-22 overexpression in human but not mouse cells.

conclusionThese data suggest that different miRNAs contribute to the regulation of PP2Cm expression in a species-specific manner. miR-204 and miR-211 are efficient in both mouse and human cells, while miR-22 regulates PP2Cm expression only in human cells.

Indexed as

Gene Expression Regulation3' Untranslated RegionsAnimalsCell LineHumansMiceMicroRNAsPhosphoprotein PhosphatasesProtein Phosphatase 2CRNA, Messenger3' Untranslated RegionsMicroRNAsMIRN204 microRNA, humanMIRN211 microRNA, humanMIRN22 microRNA, humanPhosphoprotein PhosphatasesPPM1K protein, humanProtein Phosphatase 2CRNA, Messenger

Identifiers

PMID26592513
PMCPMC4816230
OpenAlexW2173329266

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.