ArticleCurrent biology : CB2015
Warts phosphorylates mud to promote pins-mediated mitotic spindle orientation in Drosophila, independent of Yorkie.
Article in Current biology : CB, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- aPKC and F-actin dynamics promote Hippo pathway polarity in asymmetrically dividing neuroblasts.Biology open · 2026Article
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- Phase Separation and Mechanical Forces in Regulating Asymmetric Cell Division of Neural Stem Cells.International journal of molecular sciences · 2021Review
- The Hippo kinase LATS2 impairs pancreatic β-cell survival in diabetes through the mTORC1-autophagy axis.Nature communications · 2021Article
- Mud binds the kinesin-14 Ncd inBiochemistry and biophysics reports · 2021Article
- Mud Loss Restricts Yki-Dependent Hyperplasia inJournal of developmental biology · 2020Article
- A Novel Mechanism for Activation of Myosin Regulatory Light Chain by Protein Kinase C-Delta inGenetics · 2020Article
- Mob Family Proteins: Regulatory Partners in Hippo and Hippo-Like Intracellular Signaling Pathways.Frontiers in cell and developmental biology · 2020Review
- A Cell Adhesion-Based Reconstitution Method for Studying Cell Polarity.Frontiers in cell and developmental biology · 2020Article
- Junctional tumor suppressors interact with 14-3-3 proteins to control planar spindle alignment.The Journal of cell biology · 2019Article
- Oriented Cell Divisions Are Not Required for Drosophila Wing Shape.Current biology : CB · 2019Article
- The Hippo Signaling Network and Its Biological Functions.Annual review of genetics · 2018Review
- YAP regulates PD-L1 expression in human NSCLC cells.Oncotarget · 2017Article
- Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity.Molecular biology of the cell · 2017Article
- The LATS1 and LATS2 tumor suppressors: beyond the Hippo pathway.Cell death and differentiation · 2017Review
- The large tumor suppressor family: friend or foe?Journal of thoracic disease · 2017Article
- Article
- The Hippo Pathway Targets Rae1 to Regulate Mitosis and Organ Size and to Feed Back to Regulate Upstream Components Merlin, Hippo, and Warts.PLoS genetics · 2016Article
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3 authors at 1 institution in 1 country.
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Abstract
Multicellular animals have evolved conserved signaling pathways that translate cell polarity cues into mitotic spindle positioning to control the orientation of cell division within complex tissue structures. These oriented cell divisions are essential for the development of cell diversity and the maintenance of tissue homeostasis. Despite intense efforts, the molecular mechanisms that control spindle orientation remain incompletely defined. Here, we describe a role for the Hippo (Hpo) kinase complex in promoting Partner of Inscuteable (Pins)-mediated spindle orientation. Knockdown of Hpo, Salvador (Sav), or Warts (Wts) each result in a partial loss of spindle orientation, a phenotype previously described following loss of the Pins-binding protein Mushroom body defect (Mud). Similar to orthologs spanning yeast to mammals, Wts kinase localizes to mitotic spindle poles, a prominent site of Mud localization. Wts directly phosphorylates Mud in vitro within its C-terminal coiled-coil domain. This Mud coiled-coil domain directly binds the adjacent Pins-binding domain to dampen the Pins/Mud interaction, and Wts-mediated phosphorylation uncouples this intramolecular Mud interaction. Loss of Wts prevents cortical Pins/Mud association without affecting Mud accumulation at spindle poles, suggesting phosphorylation acts as a molecular switch to specifically activate cortical Mud function. Finally, loss of Wts in Drosophila imaginal disc epithelial cells results in diminished cortical Mud and defective planar spindle orientation. Our results provide new insights into the molecular basis for dynamic regulation of the cortical Pins/Mud spindle positioning complex and highlight a novel link with an essential, evolutionarily conserved cell proliferation pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.