ArticleChemistry & biology2015
Molecular Basis of Spectral Diversity in Near-Infrared Phytochrome-Based Fluorescent Proteins.
Article in Chemistry & biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 62 citations in OpenAlex.
- 3D-PAULM: Integrated Photoacoustic Tomography and Ultrasound Localization Microscopy for Multiparametric Brain and Tumor Imaging.bioRxiv : the preprint server for biology · 2026Article
- Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging.Nature methods · 2026Article
- The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Engineering a Bright Near-Infrared Fluorescent Protein by Screening a Comprehensive Phenotypic Landscape.bioRxiv : the preprint server for biology · 2025Article
- Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging.bioRxiv : the preprint server for biology · 2025Article
- Integrated Study of Fluorescence Enhancement in the Y176H Variant of Cyanobacterial Phytochrome Cph1.Biochemistry · 2025Article
- Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms.Nature communications · 2023Article
- Modulation of biliverdin dynamics and spectral properties by Sandercyanin.RSC advances · 2022Article
- Impact of Double Covalent Binding of BV in NIR FPs on Their Spectral and Physicochemical Properties.International journal of molecular sciences · 2022Article
- Enhancing the Inhomogeneous Photodynamics of Canonical Bacteriophytochrome.The journal of physical chemistry. B · 2022Article
- Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.Biochemistry · 2022Article
- Near-infrared and far-red genetically encoded indicators of neuronal activity.Journal of neuroscience methods · 2021Article
- Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome.Communications chemistry · 2021Article
- Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.Science advances · 2020Article
- A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales.Nature communications · 2020Article
- Chromophore binding to two cysteines increases quantum yield of near-infrared fluorescent proteins.Scientific reports · 2019Article
- Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing.Nature communications · 2019Article
- Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.Trends in biotechnology · 2018Review
- The Pathways of the iRFP713 Unfolding Induced by Different Denaturants.International journal of molecular sciences · 2018Article
- Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are the probes of choice for deep-tissue imaging. Detection of several processes requires spectrally distinct NIR FPs. We developed an NIR FP, BphP1-FP, which has the most blue-shifted spectra and the highest fluorescence quantum yield among BphP-derived FPs. We found that these properties result from the binding of the biliverdin chromophore to a cysteine residue in the GAF domain, unlike natural BphPs and other BphP-based FPs. To elucidate the molecular basis of the spectral shift, we applied biochemical, structural and mass spectrometry analyses and revealed the formation of unique chromophore species. Mutagenesis of NIR FPs of different origins indicated that the mechanism of the spectral shift is general and can be used to design multicolor NIR FPs from other BphPs. We applied pairs of spectrally distinct point cysteine mutants to multicolor cell labeling and demonstrated that they perform well in model deep-tissue imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.