ArticleJournal of experimental botany2016
Two key polymorphisms in a newly discovered allele of the Vitis vinifera TPS24 gene are responsible for the production of the rotundone precursor α-guaiene.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Grape Berry Secondary Metabolites and Their Modulation by Abiotic Factors in a Climate Change Scenario-A Review.Frontiers in plant science · 2021Pooled it
- Effects of Harvest Timing on Selected Aroma-Related Volatile Compounds in Blaufränkisch and Pinot Noir Wines.Foods (Basel, Switzerland) · 2026Article
- Genome-Wide Identification and Expression Analysis ofBiology · 2026Article
- Characterization and functional reconstruction of a highly productive germacrene A synthase from Liriodendron chinense.Plant biotechnology journal · 2025Article
- Identification and expression analysis ofHeliyon · 2024Article
- Plant terpene specialized metabolism: complex networks or simple linear pathways?The Plant journal : for cell and molecular biology · 2023Review
- Grapevine genome analysis demonstrates the role of gene copy number variation in the formation of monoterpenes.Frontiers in plant science · 2023Article
- Grapevine mono- and sesquiterpenes: Genetics, metabolism, and ecophysiology.Frontiers in plant science · 2023Review
- Taxonomic Insights and Its Type Cyclization Correlation of Volatile Sesquiterpenes inMolecules (Basel, Switzerland) · 2021Review
- Seeing the Forest through the (Phylogenetic) Trees: Functional Characterisation of Grapevine Terpene Synthase (Plants (Basel, Switzerland) · 2021Article
- Molecular Tools for Adapting Viticulture to Climate Change.Frontiers in plant science · 2021Review
- Germacrene A-A Central Intermediate in Sesquiterpene Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Terpene Synthases and Terpene Variation inPlant physiology · 2020Article
- Comparative (Within Species) Genomics of theFrontiers in genetics · 2020Article
- In planta and in silico characterization of five sesquiterpene synthases from Vitis vinifera (cv. Shiraz) berries.Planta · 2019Article
- Linking Terpene Synthases to Sesquiterpene Metabolism in Grapevine Flowers.Frontiers in plant science · 2019Article
- The genetic basis of grape and wine aroma.Horticulture research · 2019Review
- P450s controlling metabolic bifurcations in plant terpene specialized metabolism.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2018Article
- Distribution of Rotundone and Possible Translocation of Related Compounds Amongst Grapevine Tissues in Vitis vinifera L. cv. Shiraz.Frontiers in plant science · 2016Article
- Meta-Analysis of the Core Aroma Components of Grape and Wine Aroma.Frontiers in plant science · 2016Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Rotundone was initially identified as a grape-derived compound responsible for the peppery aroma of Shiraz wine varieties. It has subsequently been found in black and white pepper and several other spices. Because of its potent aroma, the molecular basis for rotundone formation is of particular relevance to grape and wine scientists and industry. We have identified and functionally characterized in planta a sesquiterpene synthase, VvGuaS, from developing grape berries, and have demonstrated that it produces the precursor of rotundone, α-guaiene, as its main product. The VvGuaS enzyme is a novel allele of the sesquiterpene synthase gene, VvTPS24, which has previously been reported to encode VvPNSeInt, an enzyme that produces a variety of selinene-type sesquiterpenes. This newly discovered VvTPS24 allele encodes an enzyme 99.5% identical to VvPNSeInt, with the differences comprising just 6 out of the 561 amino acid residues. Molecular modelling of the enzymes revealed that two of these residues, T414 and V530, are located in the active site of VvGuaS within 4 Å of the binding-site of the substrate, farnesyl pyrophosphate. Mutation of these two residues of VvGuaS into the corresponding polymorphisms in VvPNSeInt results in a complete functional conversion of one enzyme into the other, while mutation of each residue individually produces an intermediate change in the product profile. We have therefore demonstrated that VvGuaS, an enzyme responsible for production of the rotundone precursor, α-guaiene, is encoded by a novel allele of the previously characterized grapevine gene VvTPS24 and that two specific polymorphisms are responsible for functional differences between VvTPS24 alleles.
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