ArticleApplied and environmental microbiology2016
Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Energetic investigations of Acidianus ambivalens metabolism during anaerobic sulfur reduction and comparisons to aerobic sulfur oxidation.Extremophiles : life under extreme conditions · 2025Article
- The Lrs14 family of DNA-binding proteins as nucleoid-associated proteins in the Crenarchaeal order Sulfolobales.Molecular microbiology · 2025Review
- Membrane lipid and expression responses ofFrontiers in microbiology · 2023Article
- Physiological Characterization ofInternational journal of environmental research and public health · 2021Article
- The Cell Membrane ofInternational journal of molecular sciences · 2020Review
- Acid Experimental Evolution of the HaloarchaeonFrontiers in microbiology · 2020Article
- Participation of UV-regulated Genes in the Response to Helix-distorting DNA Damage in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius.Microbes and environments · 2019Article
- Global Transcriptional Programs in Archaea Share Features with the Eukaryotic Environmental Stress Response.Journal of molecular biology · 2019Review
- Methylation deficiency of chromatin proteins is a non-mutational and epigenetic-like trait in evolved lines of the archaeonThe Journal of biological chemistry · 2019Article
- Evidence of an Epigenetics System in Archaea.Epigenetics insights · 2019Article
- Calditol-linked membrane lipids are required for acid tolerance inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nonmutational mechanism of inheritance in the ArchaeonProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- Microbial biogeography of 925 geothermal springs in New Zealand.Nature communications · 2018Article
- In a quest for engineering acidophiles for biomining applications: challenges and opportunities.Genes · 2018Review
- Geobiological feedbacks and the evolution of thermoacidophiles.The ISME journal · 2018Article
- Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula.Journal of industrial microbiology & biotechnology · 2017Article
- Increased acid resistance of the archaeon, Metallosphaera sedula by adaptive laboratory evolution.Journal of industrial microbiology & biotechnology · 2016Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extremely thermoacidophilic Crenarchaeota belonging to the order Sulfolobales flourish in hot acidic habitats that are strongly oxidizing. The pH extremes of these habitats, however, often exceed the acid tolerance of type species and strains. Here, adaptive laboratory evolution was used over a 3-year period to test whether such organisms harbor additional thermoacidophilic capacity. Three distinct cell lines derived from a single type species were subjected to high-temperature serial passage while culture acidity was gradually increased. A 178-fold increase in thermoacidophily was achieved after 29 increments of shifted culture pH resulting in growth at pH 0.8 and 80°C. These strains were named super-acid-resistant Crenarchaeota (SARC). Mathematical modeling using growth parameters predicted the limits of acid resistance, while genome resequencing and transcriptome resequencing were conducted for insight into mechanisms responsible for the evolved trait. Among the mutations that were detected, a set of eight nonsynonymous changes may explain the heritability of increased acid resistance despite an unexpected lack of transposition. Four multigene components of the SARC transcriptome implicated oxidative stress as a primary challenge accompanying growth at acid extremes. These components included accelerated membrane biogenesis, induction of the mer operon, and an increased capacity for the generation of energy and reductant.
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