ArticleApplied and environmental microbiology2016
Defluviitalea phaphyphila sp. nov., a Novel Thermophilic Bacterium That Degrades Brown Algae.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 51 citations in OpenAlex.
- Comparative molecular dynamics simulations provided insights into the mechanisms of cold-adaption of alginate lyases from the PL7 family.Extremophiles : life under extreme conditions · 2024Article
- Construction of synthetic anti-fouling consortia: fouling control effects and polysaccharide degradation mechanisms.Microbial cell factories · 2023Article
- A Recombinant Alginate Lyase Algt1 with Potential in Preparing Alginate Oligosaccharides at High-Concentration Substrate.Foods (Basel, Switzerland) · 2023Article
- Distinct roles of carbohydrate-binding modules in multidomain β-1,3-1,4-glucanase on polysaccharide degradation.Applied microbiology and biotechnology · 2023Article
- Identification and structural analysis of a carbohydrate-binding module specific to alginate, a representative of a new family, CBM96.The Journal of biological chemistry · 2023Article
- Alginate Lyases from Marine Bacteria: An Enzyme Ocean for Sustainable Future.Molecules (Basel, Switzerland) · 2022Review
- Valorisation of algal biomass to value-added metabolites: emerging trends and opportunities.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2022Review
- Metabolic responses of thermophilic endospores to sudden heat-induced perturbation in marine sediment samples.Frontiers in microbiology · 2022Article
- Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.The ISME journal · 2021Article
- Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.Biotechnology for biofuels · 2021Review
- Importance ofMicroorganisms · 2020Article
- Microbial Community Drivers in Anaerobic Granulation at High Salinity.Frontiers in microbiology · 2020Article
- The molecular basis of endolytic activity of a multidomain alginate lyase fromThe Journal of biological chemistry · 2019Article
- Fermentation of Mannitol Extracts From Brown Macro Algae by ThermophilicFrontiers in microbiology · 2018Article
- Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila.Biotechnology for biofuels · 2016Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Brown algae are one of the largest groups of oceanic primary producers for CO2 removal and carbon sinks for coastal regions. However, the mechanism for brown alga assimilation remains largely unknown in thermophilic microorganisms. In this work, a thermophilic alginolytic community was enriched from coastal sediment, from which an obligate anaerobic and thermophilic bacterial strain, designated Alg1, was isolated. Alg1 shared a 16S rRNA gene identity of 94.6% with Defluviitalea saccharophila LIND6LT2(T). Phenotypic, chemotaxonomic, and phylogenetic studies suggested strain Alg1 represented a novel species of the genus Defluviitalea, for which the name Defluviitalea phaphyphila sp. nov. is proposed. Alg1 exhibited an intriguing ability to convert carbohydrates of brown algae, including alginate, laminarin, and mannitol, to ethanol and acetic acid. Three gene clusters participating in this process were predicted to be in the genome, and candidate enzymes were successfully expressed, purified, and characterized. Six alginate lyases were demonstrated to synergistically deconstruct alginate into unsaturated monosaccharide, followed by one uronic acid reductase and two 2-keto-3-deoxy-d-gluconate (KDG) kinases to produce pyruvate. A nonclassical mannitol 1-phosphate dehydrogenase, catalyzing D-mannitol 1-phosphate to fructose 1-phosphate in the presence of NAD(+), and one laminarase also were disclosed. This work revealed that a thermophilic brown alga-decomposing system containing numerous novel thermophilic alginate lyases and a unique mannitol 1-phosphate dehydrogenase was adopted by the natural ethanologenic strain Alg1 during the process of evolution in hostile habitats.
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