Evidence map›Paper›PMID 26589607›Full record

ArticleChinese journal of integrative medicine2016

Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation.

Yi-Xiang Song, Jun-Ye Miao, Min Qiang, Rong-Qiao He, Xue-Mei Wang, Wei-Wei Li

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Article in Chinese journal of integrative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

  1. Icariin Against Neurodegeneration: A Focus in Cell Death Pathways.International journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Yi-Xiang SongIntegrated Laboratory of Chinese and Western Medicine, Peking University First Hospital, Beijing, 100034, China.
Jun-Ye MiaoState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Min QiangState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Rong-Qiao HeState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Xue-Mei WangIntegrated Laboratory of Chinese and Western Medicine, Peking University First Hospital, Beijing, 100034, China.
Wei-Wei LiIntegrated Laboratory of Chinese and Western Medicine, Peking University First Hospital, Beijing, 100034, China. weiwei_ivy@163.com.
Chinese Academy of Sciences · CNPeking University First Hospital · CNInstitute of Biophysics · CNPeking University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the neuroprotective effects of icariin on formaldehyde (FA)-treated human neuroblastoma SH-SY5Y cells and the possible mechanisms involved.

methodsSH-SY5Y cells were divided into FA treatment group, FA treatment group with icariin, and the control group. Cell viability, apoptosis, and morphological changes were determined by cell counting kit-8 (CCK 8), flow cytometry, and confocal microscopy, respectively. The phosphorylation of Tau protein was examined by western blotting.

resultsFA showed a half lethal dose (LD50) of 0.3 mmol/L in SH-SY5Y cells under the experimental conditions. Icariin (1-10 µmol/L) prevented FA-induced cell death in SH-SY5Y cells in a dose-dependent manner, with the optimal effect observed at 5 µmol/L. After FA treatment, the absorbance in FA group was 1.31±0.05, while in the group of icariin (5 µmol/L) was 1.63±0.05. Examination of cell morphology by confocal microscopy demonstrated that 5 µmol/L icariin significantly attenuated FA-induced cell injury (P <0.05). Additionally, Icariin inhibited FA-induced cell apoptosis in SH-SY5Y cells. Results from western blotting showed that icariin suppressed FA-induced phosphorylation at Thr 181 and Ser 396 of Tau protein, while having no effect on the expression of the total Tau protein level. Furthermore, FA activated Tau kinase glycogen synthase kinase 3 beta (GSK-3β) by enhancement of Y216 phosphorylation, but icariin reduced Y216 phosphorylation and increased Ser 9 phosphorylation.

conclusionIcariin protects SH-SY5Y cells from FA-induced injury poßsibly through the inhibition of GSK-3β-mediated Tau phosphorylation.

Indexed as

Blotting, WesternCell DeathCell Line, TumorCell ShapeCell SurvivalDNA FragmentationFlavonoidsFormaldehydeGlycogen Synthase Kinase 3 betaHumansNeuroprotective AgentsPhosphorylationtau ProteinsFlavonoidsFormaldehydeGlycogen Synthase Kinase 3 betaicariinNeuroprotective Agentstau Proteinsformaldehydeglycogen synthase kinase 3 betaicariinneuroprotectionTau phosphorylation

Identifiers

PMID26589607
OpenAlexW2262881704

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.