Evidence map›Paper›PMID 26589597›Full record

ArticleAnnals of biomedical engineering2016

Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients.

Maggie A Ostrowski, Eva Y Huang, Vinay N Surya, Charlotte Poplawski, Joseph M Barakat, Gigi L Lin, Gerald G Fuller, Alexander R Dunn

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Maggie A OstrowskiDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Eva Y HuangDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Vinay N SuryaDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Charlotte PoplawskiDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Joseph M BarakatDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Gigi L LinDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Gerald G FullerDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA.
Alexander R DunnDepartment of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA. alex.dunn@stanford.edu.
Stanford University · US

Funding

Uncovering New Roles for Mechanical Force in Tissue Development and RemodelingDP2OD007078 · OD · STANFORD UNIVERSITY · PI DUNN, ALEXANDER R · 2010 to 2010
$2.4M
Molecular mechanisms underlying flow sensing in lymphatic endothelial cellsR01HL128779 · NHLBI · STANFORD UNIVERSITY · PI DUNN, ALEXANDER R, FULLER, GERALD · 2015 to 2018
$1.5M
NHLBI NIH HHS R01 HL128779NIH HHS DP2 OD007078Wellcome Trust
6 · The paper itself

Abstract

Endothelial cells (ECs) line the interior of blood and lymphatic vessels and experience spatially varying wall shear stress (WSS) as an intrinsic part of their physiological function. How ECs, and mammalian cells generally, sense spatially varying WSS remains poorly understood, due in part to a lack of convenient tools for exposing cells to spatially varying flow patterns. We built a multiplexed device, termed a 6-well impinging flow chamber, that imparts controlled WSS gradients to a six-well tissue culture plate. Using this device, we investigated the migratory response of lymphatic microvascular ECs, umbilical vein ECs, primary fibroblasts, and epithelial cells to WSS gradients on hours to days timescales. We observed that lymphatic microvascular ECs migrate upstream, against the direction of flow, a response that was unique among all the cells types investigated here. Time-lapse, live cell imaging revealed that the microtubule organizing center relocated to the upstream side of the nucleus in response to the applied WSS gradient. To further demonstrate the utility of our device, we screened for the involvement of canonical signaling pathways in mediating this upstream migratory response. These data highlight the importance of WSS magnitude and WSS spatial gradients in dictating the cellular response to fluid flow.

Indexed as

Cell MovementMechanotransduction, CellularShear StrengthStress, MechanicalEndothelial CellsHumansHuman Umbilical Vein Endothelial CellsCell migrationFluid mechanicsMechanotransductionVascular biology

Identifiers

PMID26589597
PMCPMC4874920
OpenAlexW2262356990

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.